This study evaluated a work readiness HIV peer worker training and internship program in New York City and found that participation significantly decreased depression and internalized HIV stigma, while significantly increasing self-esteem, medication adherence, and patient self-advocacy. Among 137 participants who completed the training and 55 who also completed a six-month paid internship, psychosocial and health-related behavioral improvements were observed and maintained through the internship period. These findings suggest that peer worker training programs serve not only as vocational interventions but also as tools to improve psychosocial and health outcomes for people living with HIV.