**Background:** Old wheat varieties and local landraces are gaining consumer attention for their potential nutritional and sensory advantages over modern high-yielding wheats, but they typically have weak gluten and poor technological properties for breadmaking. 'Avanzi 3-Grano 23' (G23) is an old *Triticum aestivum* variety from Lunigiana, Italy, that is now endangered due to the dominance of modern wheat cultivation. G23 flour has desirable nutritional and sensory traits but limited baking suitability. This study aimed to optimize bread formulation using G23 by combining flour blending with different leavening systems (sourdough vs. baker's yeast) to enhance its technological, sensory, and nutritional performance.
**Methods:** Preliminary leavening tests compared three G23-to-strong-flour (C) ratios — M1 (1:3 w/w), M2 (1:1 w/w), and M3 (3:1 w/w) — using either sourdough (S) or baker's yeast (Y). Dough volume increase (DVI) and fermentation metabolites (acetic acid, lactic acid, ethanol) were measured. The M2 blend (1:1) was selected for further breadmaking trials, with 100% C flour as control. Four bread types were produced: sourdough bread with M2 (SB-M2), sourdough bread with C (SB-C), baker's yeast bread with M2 (YB-M2), and baker's yeast bread with C (YB-C). Breads were characterized for moisture, pH, total titratable acidity (TTA), softness, water activity, color (CIE L*a*b*), total polyphenols (Folin–Ciocalteu), total flavonoids, antioxidant activity (DPPH, ABTS, FRAP), volatile organic compounds (SPME-GC-MS), and sensory profile (8 trained panelists, quantitative and hedonic indices).
**Key Results:** In preliminary tests, M2 (1:1) allowed the greatest G23 inclusion with acceptable DVI and metabolite production for both leavening systems. For breads, SB-M2 showed the highest softness (penetration units not explicitly reported in the text) and significantly higher acidity (TTA and pH) than SB-C, indicating better sourdough substrate utilization. Phytochemically, control breads (C flours) had higher absolute polyphenol and antioxidant values due to higher initial content, but SB-M2 showed the greatest relative increase from its starting flour values, attributed to sourdough's release of bound phenolics. YB-M2 and YB-C showed lower phytochemical values than their starting flours. Color analysis showed perceptible differences among all samples (ΔE*ab range not fully specified; greatest distance 8.89 between SB-M2 and YB-C). VOC analysis identified 62 compounds; sourdough breads had more complex profiles with pyrazines, pyrimidines, and carboxylic acids, while baker's yeast breads were dominated by alcohols and aldehydes. SB-M2 was characterized by aldehydes (e.g., (Z)-2-heptanal, benzaldehyde), pyrazines (2,6-dimethylpyrazine, methoxypyrazine), and monoterpenes (p-cymene). Sensory evaluation showed SB-M2 received the highest hedonic index (HI > 8), followed by both baker's yeast breads (HI > 6). Sourdough breads had higher perceived acidity, with SB-M2 scoring highest. M2 flour breads were evaluated more positively than C flour breads regardless of leavening agent.
**Clinical Implications:** This is a food science study with no direct clinical implications. However, the findings support the development of functional bakery products with improved antioxidant capacity and phenolic bio-accessibility through sourdough fermentation of old wheat varieties. The higher acidity of sourdough bread may prolong shelf-life naturally. The study demonstrates a viable strategy for valorizing endangered local wheat varieties, supporting biodiversity, local economies, and short supply chains.