This cross-sectional study surveyed 145 hospital-based health professionals in Italy to assess their needs and expectations for an mHealth app supporting the first 1000 days of life. Health professionals strongly valued app content on pregnancy health (92% rated very important/essential) and infections during pregnancy (91%), while expressing skepticism about social media integration and tracking features. The findings support a participatory co-design approach, with most professionals (63.4%) falling into a 'medium demanding' cluster, indicating general readiness to integrate such an app into clinical practice.