**Background:** Work stress imposes a heavy economic and disease burden, and digital stress management interventions (dSMIs) offer scalable solutions. However, ethical risks—especially those involving biomedical big data and machine learning—are often overlooked. Value Sensitive Design (VSD) is a framework that front-loads ethics by systematically accounting for human values throughout technology design. This study aimed to identify relevant values for dSMIs at the workplace, assess how employees comprehend those values, and derive ethics-informed design requirements.
**Methods:** A narrative literature search identified relevant values. A web-based study was then conducted with employees of a large Swiss insurance company. Participants watched a 3-minute video introducing either a dSMI with a JITAI component (using ML-based monitoring of physiological/behavioral data) or a dSMI without monitoring. They then completed closed-ended scales measuring value-related concerns, wishes, user acceptance, and health trade-offs, plus open-ended questions. Quantitative analysis used nonparametric tests (Mann-Whitney U, Kruskal-Wallis) with Cliff delta effect sizes; qualitative data were analyzed using framework method analysis (FMA).
**Key Results:** Of 241 participants, 170 were included in the final analysis (70 non-JITAI, 100 JITAI). Mean intention to use was 4.60 (SD 1.66) and perceived usefulness was 4.12 (SD 1.40) on a 7-point scale. Health and well-being concerns were moderate (beneficence: mean 3.63, SD 1.51; nonmaleficence: mean 4.19, SD 1.62). Privacy concerns were moderate-to-high (mean 3.69, SD 1.81), while autonomy (mean 2.63, SD 1.32), identity (mean 2.14, SD 1.37), and accountability (mean 2.91, SD 1.24) concerns were lower. The JITAI dSMI led to significantly higher privacy concerns (median 4.33 vs 2.83; U=4322.5, P=.009, δ=0.24) and accountability concerns (median 2.67 vs 2.33; U=4145, P=.04, δ=0.18) compared to the non-JITAI dSMI. Employees with low propensity to trust health technologies had significantly higher accountability concerns (median 3.00 vs 2.33; U=4359, P=.02, δ=0.22) and beneficence concerns (median 4.00 vs 3.25; U=4576.5, P=.002, δ=0.28). Employees with severe/extremely severe stress had significantly higher nonmaleficence concerns than those with normal stress (median 5.33 vs 4.00; P=.02, δ=0.46). Qualitative analysis of 85 text responses from 70 participants confirmed all five identified values and revealed three novel values: integrability, digital independence, and user-friendliness. Over 40% of participants were completely unwilling to share eye movement or body posture data.
**Clinical Implications:** The findings highlight that while employees are generally open to dSMIs, significant ethical concerns must be addressed—particularly regarding privacy and accountability when ML-based monitoring is involved. The authors recommend data minimization, client-side processing, transparent consent, adjustable privacy settings, and the option to deactivate monitoring. For a minority valuing digital independence, non-digital stress management alternatives should be offered. These VSD-informed requirements aim to align dSMI design with employee values, potentially improving uptake, adherence, and ethical integrity of workplace digital health interventions.