A Comprehensive Assessment of Tear-Film-Oriented Diagnosis (TFOD) in a Dacryoadenectomy Dry Eye Model

International Journal of Molecular Sciences · 14 authors, 9 centres

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TL;DR

This preclinical study developed and characterized a rabbit model of dry eye by removing lacrimal glands, finding it exhibited tear film instability similar to a common human dry eye type. The key result was a significant and stable shortening of tear film breakup time (TBUT) for up to 8 weeks post-surgery.

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The study evaluated a rabbit dry eye model induced by surgical removal of the lacrimal glands (dacryoadenectomy). The central finding was a significant and consistent shortening of TBUT for 8 weeks post-surgery, alongside an increased blink rate and dimple/random breakup patterns. The model did not show significant changes in aqueous tear volume (Schirmer’s I test) or histology. The authors conclude this model partially captures the phenotype of human short TBUT-type dry eye and may be useful for drug development studies.

STUDY TYPE

other

SPECIALTY

ophthalmology

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ORIGINAL PAPER

PMC10671533

A Comprehensive Assessment of Tear-Film-Oriented Diagnosis (TFOD) in a Dacryoadenectomy Dry Eye Model

International Journal of Molecular Sciences

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ABSTRACT

Tear film instability is a major cause of dry eye disease. In order to treat patients with short tear film breakup time (TBUT)-type dry eye, the development of tear film stabilizing agents is essential. However, the lack of an appropriate animal model of tear film instability has made drug development difficult. Although rabbit dry eye models have been reported in the past, there are only a few reports that focus on tear film instability. Herein, we assessed the tear film stability of a rabbit dry eye model induced by dacryoadenectomy. A clinical evaluation of the ocular surface, interferometry, and histological assessments of the cornea and conjunctiva were performed. Following the removal of the lacrimal glands, TBUT was shortened significantly, with dimple and random breakup patterns prominently observed. Furthermore, the blink rate in this model increased after dacryoadenectomy, suggesting that this model partially captured the phenotypes of human short TBUT-type dry eye and may be useful as an animal model for investigating potential drug candidates.

CITATION EXPORT

Saki Sakakura, Emi Inagaki, Yuichiro Ochiai et al.. A Comprehensive Assessment of Tear-Film-Oriented Diagnosis (TFOD) in a Dacryoadenectomy Dry Eye Model. International Journal of Molecular Sciences. (2023). https://doi.org/10.3390/ijms242216510

AUTHORS

14 · 9 CENTRES

Saki Sakakura, Emi Inagaki, Yuichiro Ochiai, Masatoshi Yamamoto, Naofumi Takai, Taeko Nagata, Kazunari Higa, Yasunori Sato, Hiroshi Toshida, Dogru Murat, Masatoshi Hirayama, Yoko Ogawa, Kazuno Negishi, Shigeto Shimmura

1Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, [email protected] (M.H.); [email protected] (K.N.)
2Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
3Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan
4Kitayama Labes Co., Ltd., 3052-1 Arai, Ina City 396-0025, Japan
5Cornea Center and Eye Bank, Tokyo Dental College Ichikawa General Hospital, 5-11-13 Sugano, Ichikawa 272-8513, Japan; [email protected]
6Department of Preventive Medicine and Public Health, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; [email protected]
7Department of Ophthalmology, Juntendo University Shizuoka Hospital, Nagaoka 1129, Izunokuni City 410-2295, Japan; [email protected]
8Department of Ophthalmology, Tsurumi University, 2-1-3 Tsurumi, Yokohama 230-0063, Japan
9Department of Clinical Regenerative Medicine, Fujita Medical Innovation Center, Fujita Health University, Haneda Innovation City Zone A, 1-1-4 Hanedakuko, Ota-ku, Tokyo 144-0041, Japan

EDITOR / REVIEWER

J. Fielding Hejtmancik