Molecules · 3 authors, 2 centres
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This study developed a simple colorimetric method using resazurin to assess antioxidant activity in gelatin biopolymer films. The method can track lipid oxidation via color changes, but it has limitations in detecting certain antioxidant mechanisms like metal chelation.
Gelatin-based biopolymer films are used for active packaging with antioxidants to prevent lipid oxidation. Traditional methods for measuring antioxidant activity are complex, so a simple method is needed. Methods: The study created a model food system (AES-R) with agar, soybean oil, emulsifier, and resazurin as a pH indicator. Gelatin biopolymer films (GBFs) incorporated with antioxidants (ascorbic acid, BHA, phytic acid) at concentrations of 0.2%, 0.6%, and 1.0% were tested. Mechanical properties, water vapor permeability, oxygen permeability, and color changes (brightness and redness) were measured over 14 days. DPPH radical scavenging tests were also performed. Key Results: Phytic acid improved mechanical properties and reduced water vapor permeability. BHA-based GBFs showed slow color change in the AES-R system, indicating antioxidant activity. Ascorbic acid at high concentrations acted as a prooxidant. The method correlated with DPPH tests but had inconsistencies due to solvent differences. Limitations: The AES-R system lacks metallic ions, so it may not detect chelating antioxidants like phytic acid effectively. Color changes can be influenced by oil color and prooxidant effects. Implications: The method is simple, rapid, and low-cost for measuring antioxidant activity in food packaging, but further modifications are needed for broader application.
