A possible genetic association between obesity and colon cancer in females

Frontiers in Endocrinology · 8 authors, 4 centres

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TL;DR

This bioinformatics study analyzed public gene expression data from female obesity and colon cancer patients. It identified 146 shared differentially expressed genes, primarily in inflammatory and immune pathways, and highlighted 14 hub genes with associated miRNAs and transcription factors.

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This study used bioinformatics to explore a potential genetic link between obesity and colon cancer in females by analyzing two public GEO microarray datasets (GSE44076 and GSE199063). Researchers identified 146 differentially expressed genes (DEGs) common between female colon cancer patients and female obesity patients, which were enriched in inflammatory and immune-related pathways. Through network analysis, they identified 14 hub genes (e.g., TYROBP, CD44, IL6) and constructed regulatory networks involving key transcription factors (WRNIP1, ATF1, CBFB, NR2F6) and microRNAs (hsa-mir-1-3p, hsa-mir-26b-5p). The analysis suggests these shared genes and networks may contribute to disease development via endocrine disruption and chronic inflammation, especially in postmenopausal women. Limitations include the lack of a dedicated dataset for colon cancer within obese females and the absence of experimental validation for the computational findings.

STUDY TYPE

other

SPECIALTY

oncology

SUMMARISED BY

AI pipeline

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100% · A

ORIGINAL PAPER

PMC10497871

A possible genetic association between obesity and colon cancer in females

Frontiers in Endocrinology

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ABSTRACT

Object There is mounting clinical evidence that an increase in obesity is linked to an increase in cancer incidence and mortality. Although studies have shown a link between obesity and colon cancer, the particular mechanism of the interaction between obesity and colon cancer in females remains unknown. The goal of this work is to use bioinformatics to elucidate the genetic link between obesity and colon cancer in females and to investigate probable molecular mechanisms. Methods GSE44076 and GSE199063 microarray datasets were obtained from the Gene Expression Omnibus (GEO) database. In the two microarray datasets and healthy controls, the online tool GEO2R was utilized to investigate the differential genes between obesity and colon cancer. The differential genes (DEGs) identified in the two investigations were combined. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment studies were performed on the DEGs. The STRING database and Cytoscape software were then used to build protein-protein interaction (PPI) networks to discover hub genes. NetworkAnalyst was also used to build networks of target microRNAs (miRNAs) and hub genes, as well as networks of transcriptions. Results Between the two datasets, 146 DEGs were shared. The DEGs are primarily enriched in inflammatory and immune-related pathways, according to GO analysis and KEGG. 14 hub genes were identified via PPI building using the Cytoscape software’s MCODE and CytoNCA plug-ins: TYROBP, CD44, BGN, FCGR3A, CD53, CXCR4, FN1, SPP1, IGF1, CCND1, MMP9, IL2RG, IL6 and CTGF. Key transcription factors for these hub genes include WRNIP1, ATF1, CBFB, and NR2F6. Key miRNAs for these hub genes include hsa-mir-1-3p, hsa-mir-26b-5p, hsa-mir-164a-5p and hsa-mir-9-5p. Conclusion Our research provides evidence that changed genes are shared by female patients with colon cancer and obesity. Through pathways connected to inflammation and the immune system, these genes play significant roles in the emergence of both diseases. We created a network between hub genes and miRNAs that target transcription factors, which may offer suggestions for future research in this area.

CITATION EXPORT

Xiao-li Zhang, Xin-feng Zhang, Yuan Fang et al.. A possible genetic association between obesity and colon cancer in females. Frontiers in Endocrinology. (2023). https://doi.org/10.3389/fendo.2023.1189570

AUTHORS

8 · 4 CENTRES

Xiao-li Zhang, Xin-feng Zhang, Yuan Fang, Meng-li Li, Ruo Shu, Yi Gong, Hua-you Luo, Yan Tian

1 Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, China
2 Department of Hepatobiliary Transplantation, The First Affiliated Hospital of Kunming Medical University, Kunming, China
3 Department of Respiratory Medicine, Affiliated Hospital of Yunnan University, Kunming, China
4 Basic Medical Sciences, Kunming Medical University, Kunming, China