SEARCHNARRATIVE_REVIEWbiomedical research, preclinical research

narrative_review·biomedical research, preclinical research, research methods, veterinary medicine, gastroenterology·PMC10815683

Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm

International Journal of Molecular Sciences · 19 authors, 9 centres

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TL;DR

This narrative review describes the development and potential of organoid cultures derived from the tissues of adult veterinary and exotic animals (e.g., pig, dog, rabbit) as improved in vitro models for biomedical research within a One Health framework.

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It highlights that traditional 2D cell lines are limited and that animal-derived organoids can mimic organ epithelial structure and function better. The paper summarizes specific examples of organoid culture methods and characterization, such as canine intestinal organoids used for drug permeability studies and porcine enteroids for inflammation models. It emphasizes the potential of these models for translational and reverse translational research under the One Health paradigm, which aims to connect human, animal, and environmental health.

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narrative_review

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biomedical research

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ORIGINAL PAPER

PMC10815683

Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm

International Journal of Molecular Sciences

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ABSTRACT

Preclinical biomedical research is limited by the predictiveness of in vivo and in vitro models. While in vivo models offer the most complex system for experimentation, they are also limited by ethical, financial, and experimental constraints. In vitro models are simplified models that do not offer the same complexity as living animals but do offer financial affordability and more experimental freedom; therefore, they are commonly used. Traditional 2D cell lines cannot fully simulate the complexity of the epithelium of healthy organs and limit scientific progress. The One Health Initiative was established to consolidate human, animal, and environmental health while also tackling complex and multifactorial medical problems. Reverse translational research allows for the sharing of knowledge between clinical research in veterinary and human medicine. Recently, organoid technology has been developed to mimic the original organ’s epithelial microstructure and function more reliably. While human and murine organoids are available, numerous other organoids have been derived from traditional veterinary animals and exotic species in the last decade. With these additional organoid models, species previously excluded from in vitro research are becoming accessible, therefore unlocking potential translational and reverse translational applications of animals with unique adaptations that overcome common problems in veterinary and human medicine.

CITATION EXPORT

Vojtech Gabriel, Christopher Zdyrski, Dipak K. Sahoo et al.. Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm. International Journal of Molecular Sciences. (2024). https://doi.org/10.3390/ijms25020701

AUTHORS

19 · 9 CENTRES

Vojtech Gabriel, Christopher Zdyrski, Dipak K. Sahoo, Abigail Ralston, Hannah Wickham, Agnes Bourgois-Mochel, Basant Ahmed, Maria M. Merodio, Karel Paukner, Pablo Piñeyro, Jamie Kopper, Eric W. Rowe, Jodi D. Smith, David Meyerholz, Amir Kol, Austin Viall, Mohamed Elbadawy, Jonathan P. Mochel, Karin Allenspach

1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA; [email protected] (H.W.); [email protected] (B.A.); [email protected] (J.P.M.)
23D Health Solutions Inc., Ames, IA 50010, USA; [email protected] (C.Z.); [email protected] (A.R.); [email protected] (M.M.M.)
3Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA 50011, USA; [email protected] (D.K.S.); [email protected] (A.B.-M.); [email protected] (J.K.)
4Atherosclerosis Research Laboratory, Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, 14021 Prague, Czech Republic; [email protected]
5Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA; [email protected] (P.P.); [email protected] (J.D.S.)
6Department of Pathology, University of Iowa, Iowa City, IA 52242, USA; [email protected]
7Department of Pathology, University of California, Davis, CA 94143, USA; [email protected] (A.K.); [email protected] (A.V.)
8Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30530, USA; [email protected]
9Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt

EDITOR / REVIEWER

Sun-Woong Kang