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other·pediatrics, gastroenterology, preclinical research, biomedical research·PMC10568644

Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

Frontiers in Microbiology · 6 authors, 6 centres

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The study also identified activation of gene pathways associated with intestinal fibrosis in the ileum tissue of the PSIO mice.

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Fecal and ileal tissue samples were analyzed using 16S rRNA gene sequencing and RNA sequencing. The study is limited by its small, preclinical mouse model and does not measure microbial metabolites like SCFAs. The findings suggest PSIO-induced dysbiosis may be linked to complications such as intestinal fibrosis, but the causal mechanisms require further exploration.

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other

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pediatrics

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ORIGINAL PAPER

PMC10568644

Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

Frontiers in Microbiology

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ABSTRACT

Abstract Introduction Changes in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model. Methods A PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups. Results Bacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae ( p = 0.008) and Akkermansia ( p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella ( p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group. Conclusion PSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future. Keywords: pediatrics, partial small intestinal obstruction, gut microbiota, depleted diversity, intestinal fibrosis

CITATION EXPORT

Yong Wang, Minzhong Zhang, Lu Jiang et al.. Alterations of gut microbiota in a mouse model with partial small intestinal obstruction. Frontiers in Microbiology. (2023). https://doi.org/10.3389/fmicb.2023.1242650

AUTHORS

6 · 6 CENTRES

Yong Wang, Minzhong Zhang, Lu Jiang, Yiming Gong, Keqiang Liu, Tian Zhang

1Department of Pediatric Surgery, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
2Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangzhou, China
3Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangzhou, China
4Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China
5Department of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
6Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China