Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Toxins · 14 authors, 5 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

The study investigated the roles of two proteins, Pxpolycalin and PxABCC2, in the susceptibility of the diamondback moth (Plutella xylostella) to the Cry1Ac toxin.

Full summary

775 CHARS

The study investigated the roles of two proteins, Pxpolycalin and PxABCC2, in the susceptibility of the diamondback moth (Plutella xylostella) to the Cry1Ac toxin. Analysis showed that transcript levels of Pxpolycalin were higher in a susceptible strain than in a resistant strain. Genetic linkage analysis indicated that the Pxpolycalin gene was not linked to Cry1Ac resistance, whereas the PxABCC2 gene was linked. When treated with 0.50 μg/mL Cry1Ac toxin, homozygous knockout strains for Pxpolycalin or PxABCC2 showed decreased mortality compared to the control strain, with the PxABCC2 knockout showing a much larger reduction. The authors conclude that PxABCC2 may function as a Bt toxin receptor, while Pxpolycalin may play a role in resistance through other pathways.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10145054

Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Toxins

READ THE FULL PAPER

ABSTRACT

Abstract Many insects, including the Plutella xylostella (L.), have developed varying degrees of resistance to many insecticides, including Bacillus thuringiensis ( Bt ) toxins, the bioinsecticides derived from Bt . The polycalin protein is one of the potential receptors for Bt toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of P. xylostella , but whether polycalin is associated with the resistance of Bt toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the Pxpolycalin gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of Pxpolycalin showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the Pxpolycalin gene and its transcript level were not linked to Cry1Ac resistance, whereas both the PxABCC2 gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the Pxpolycalin gene in a short term. Furthermore, the knockout of polycalin and ATP-binding cassette transporter subfamily C2 (ABCC2) genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to Bt toxins. Keywords: polycalin, ABCC2, Bacillus thuringiensis , CRISPR/Cas9

CITATION EXPORT

Lei Xiong, Zhaoxia Liu, Jingge Li et al.. Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout. Toxins. (2023). https://doi.org/10.3390/toxins15040273

AUTHORS

14 · 5 CENTRES

Lei Xiong, Zhaoxia Liu, Jingge Li, Shuyuan Yao, Zeyun Li, Xuanhao Chen, Lingling Shen, Zhen Zhang, Yongbin Li, Qing Hou, Yuhang Zhang, Minsheng You, Zhiguang Yuchi, Shijun You

1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China
4College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China
5School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China