Scientific Reports · 8 authors, 3 centres
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This in vitro study assessed linoelaidic acid isolated from marine Tapra fish oil on MCF-7 human breast cancer cells, finding that the compound reduced cell viability in a dose-dependent manner while inducing apoptosis through ROS generation, caspase activation, redox imbalance, and p53 upregulation.
Linoelaidic acid isolated from marine Tapra fish oil was evaluated for anticancer activity against MCF-7 human breast cancer cells using MTT assay, ELISA, Western blotting, fluorescent staining (AO/Et-Br and DAPI), and biochemical assays. Treatment with 5 µM/ml linoelaidic acid for 24 h significantly reduced MCF-7 cell viability in a dose- and time-dependent manner with minimal toxicity to peripheral blood lymphocytes. The compound increased TNF-α and IL-1ra levels, decreased GSH and increased GSSG (indicating redox imbalance), elevated MDA levels (indicating lipid peroxidation), and enhanced NO production. Linoelaidic acid upregulated p53 and Bax expression, activated caspase-3 and caspase-9, and significantly increased ROS generation. AO/Et-Br and DAPI staining confirmed apoptotic morphological changes including chromatin condensation and nuclear disintegration.
