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other·preclinical research, biomedical research, dermatology, infectious disease·PMC10532769

Anti-Yeasts, Antioxidant and Healing Properties of Henna Pre-Treated by Moist Heat and Molecular Docking of Its Major Constituents, Chlorogenic and Ellagic Acids, with Candida albicans and Geotrichum candidum Proteins

Life · 4 authors, 3 centres

AI SUMMARY

FIDELITY 100%

POPULATIONNot applicable (in vitro study using yeast cultures and rat wound model)

INTERVENTIONUnpre-treated henna extract (UPMH)

COMPARISONPre-treated henna extract by moist heat (PMH)

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

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TL;DR

This preclinical study evaluated unpre-treated (UPMH) and pre-treated (PMH) henna powder, finding that UPMH had higher concentrations of key phytochemicals and demonstrated superior antifungal activity, antioxidant capacity, and in vitro wound healing compared to PMH.

Full summary

741 CHARS

This study investigated the effect of moist heat pre-treatment on henna (Lawsonia inermis) powder, comparing unpre-treated (UPMH) and pre-treated (PMH) extracts. HPLC analysis showed UPMH had higher concentrations of key flavonoids and phenolics, including chlorogenic and ellagic acids. In vitro antifungal assays against Candida albicans, C. tropicalis, and Geotrichum candidum showed UPMH had larger inhibition zones and lower MIC/MFC values than PMH. An in vitro wound healing assay using a rat model indicated UPMH promoted greater cell migration and wound closure. Both extracts showed antioxidant activity, with UPMH having a lower IC50. The central finding is that moist heat pre-treatment reduces the biological activities of henna.

PICO

PPOPULATION

Not applicable (in vitro study using yeast cultures and rat wound model)

IINTERVENTION

Unpre-treated henna extract (UPMH)

OOUTCOME

Antifungal activity (inhibition zones, MIC, MFC), antioxidant activity (IC50), in vitro wound healing (migration, closure)

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10532769

Anti-Yeasts, Antioxidant and Healing Properties of Henna Pre-Treated by Moist Heat and Molecular Docking of Its Major Constituents, Chlorogenic and Ellagic Acids, with Candida albicans and Geotrichum candidum Proteins

Life

READ THE FULL PAPER

ABSTRACT

Lawsonia inermis , known as henna, has traditionally been utilized in cosmetics and folk medicine because of their valuable health effects. A lack of information about the processes that increase or decrease release, as well as the biological activities of constituents of natural origin, is an important pharmacological problem. This investigation evaluates the influence of moist heat on the flavonoid and phenolic contents of henna powder and their biological activities. HPLC analysis reflected the existence of 20 and 19 compounds of flavonoids and phenolics in the extract of unpre-treated henna by moist heat (UPMH) and pre-treated henna by moist heat (PMH). Several compounds such as chlorogenic acid, ellagic acid, rutin, rosmarinic acid, kaempferol, and pyrocatechol occurred with high concentrations of 57,017.33, 25,821.09, 15,059.88, 6345.08, 1248.42, and 819.19 µg/mL UPMH while occurred with low concentrations of 44,286.51, 17,914.26, 3809.85, 5760.05, 49.01, and 0.0 µg/mL, respectively in PMH. C. albicans , C. tropicalis , and G. candidum were more affected by UPMH with inhibition zones of 30.17 ± 0.29, 27 ± 0.5, and 29 ± 1.5 mm than PMH with inhibition zones of 29 ± 0.5, 25.33 ± 0.58, and 24.17 ± 0.29 mm, respectively. UPMH henna exhibited less MIC and MFC against the tested yeasts than PMH. Moreover, UPMH henna showed good wound healing, where the rat of migration, wound closure %, and area difference % were 14.806 um, 74.938 um 2 , and 710.667% compared with PMH henna 11.360 um, 59.083 um 2 , 545.333%, respectively. Antioxidant activity of UPMH and PMH henna. Promising antioxidant activity was recorded for both UPMH or PMH henna with IC 50 5.46 µg/mL and 7.46 µg/mL, respectively. The docking interaction of chlorogenic acid and ellagic acid with the crystal structures of G. candidum (4ZZT) and C. albicans (4YDE) was examined. The biological screening demonstrated that the compounds had favorable docking results with particular proteins. Chlorogenic acid had robust behavior in the G. candidum (4ZZT) active pocket and displayed a docking score of −7.84379 Kcal/mol, higher than ellagic acid’s −6.18615 Kcal/mol.

CITATION EXPORT

Sulaiman A. Alsalamah, Mohammed Ibrahim Alghonaim, Mohammed Jusstaniah et al.. Anti-Yeasts, Antioxidant and Healing Properties of Henna Pre-Treated by Moist Heat and Molecular Docking of Its Major Constituents, Chlorogenic and Ellagic Acids, with Candida albicans and Geotrichum candidum Proteins. Life. (2023). https://doi.org/10.3390/life13091839

AUTHORS

4 · 3 CENTRES

Sulaiman A. Alsalamah, Mohammed Ibrahim Alghonaim, Mohammed Jusstaniah, Tarek M. Abdelghany

1Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia; [email protected] (S.A.A.); [email protected] (M.I.A.)
2University Medical Service Center, Building 70, King Abdulaziz University, Jeddah 21589, Saudi Arabia; [email protected]
3Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11725, Egypt

EDITOR / REVIEWER

Seung Ho Lee