Abstract Determining the antioxidant abilities and enzyme inhibition profiles of medicinally important plants and their oils is of great importance for a healthy life and the treatment of some common global diseases. Kiwifruit ( Actinidia deliciosa ) oil was examined and researched using several bioanalytical methods comprehensively for the first time in this research to determine its antioxidant, antiglaucoma, antidiabetic and anti-Alzheimer’s capabilities. Additionally, the kiwifruit oil inhibitory effects on acetylcholinesterase (AChE), carbonic anhydrase II (CA II), and α-amylase, which are linked to a number of metabolic illnesses, were established. Furthermore, LC-HRMS analysis was used to assess the phenolic content of kiwifruit oil. It came to light that kiwifruit oil contained 26 different phenolic compounds. According to the LC-HRMS findings, kiwifruit oil is abundant in apigenin (74.24 mg/L oil), epigallocatechin (12.89 mg/L oil), caryophyllene oxide (12.89 mg/L oil), and luteolin (5.49 mg/L oil). In addition, GC-MS and GC-FID studies were used to ascertain the quantity and chemical composition of the essential oils contained in kiwifruit oil. Squalene (53.04%), linoleoyl chloride (20.28%), linoleic acid (2.67%), and palmitic acid (1.54%) were the most abundant compounds in kiwifruit oil. For radical scavenging activities of kiwifruit oil, 1,1-diphenyl-2-picryl-hydrazil (DPPH • ) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS •+ ) radicals scavenging techniques were examined. These methods effectively demonstrated the potent radical scavenging properties of kiwifruit oil (IC 50 : 48.55 μg/mL for DPPH • , and IC 50 : 77.00 μg/mL for ABTS •+ scavenging). Also, for reducing capabilities, iron (Fe 3+ ), copper (Cu 2+ ), and Fe 3+ -2,4,6-tri(2-pyridyl)-S-triazine (TPTZ) reducing abilities were studied. Moreover, kiwifruit oil showed a considerable inhibition effect towards hCA II (IC 50 : 505.83 μg/mL), AChE (IC 50 : 12.80 μg/mL), and α-amylase (IC 50 : 421.02 μg/mL). The results revealed that the use of kiwifruit oil in a pharmaceutical procedure has very important effects due to its antioxidant, anti-Alzheimer, antidiabetic, and antiglaucoma effects. Keywords: Actinidia deliciosa , polyphenol, kiwifruit oil, antioxidant activity, LC-HRMS, GC-MS, enzyme inhibition
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Eda Mehtap Ozden, Zeynebe Bingol, Muzaffer Mutlu et al.. Antioxidant, Antiglaucoma, Anticholinergic, and Antidiabetic Effects of Kiwifruit (Actinidia deliciosa) Oil: Metabolite Profile Analysis Using LC-HR/MS, GC/MS and GC-FID. Life. (2023). https://doi.org/10.3390/life13091939 Eda Mehtap Ozden, Zeynebe Bingol, Muzaffer Mutlu, Hasan Karagecili, Ekrem Köksal, Ahmet C Goren, Saleh H Alwasel, İlhami Gulcin
1Department of Chemistry, Faculty of Science, Ataturk University, Erzurum 25240, Türkiye; [email protected] 2Department of Medical Services and Techniques, Tokat Vocational School of Health Services, Gaziosmanpasa University, Tokat 60250, Türkiye; [email protected] 3Vocational School of Applied Sciences, Gelisim University, Istanbul 34315, Türkiye; [email protected] 4Department of Nursing, Faculty of Health Sciences, Siirt University, Siirt 56100, Türkiye 5Department of Chemistry, Faculty of Science and Arts, Erzincan Binali Yildirim University, Erzincan 24100, Türkiye; [email protected] 6Department Chemistry, Faculty of Sciences, Gebze Technical University, Kocaeli 41400, Türkiye; [email protected] 7Department of Zoology, College of Science, King Saud University, Riyadh 11362, Saudi Arabia; [email protected] 