Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS

Mikrochimica Acta · 5 authors, 3 centres

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TL;DR

The method was validated for linearity, limits of detection and quantification, and precision.

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239 CHARS

The method was validated for linearity, limits of detection and quantification, and precision. Limitations include the lengthy incubation and elution times of more than 1 hour, though high throughput can be achieved using multiple vessels.

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other

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food science

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AI pipeline

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ORIGINAL PAPER

PMC10182934

Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS

Mikrochimica Acta

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ABSTRACT

Abstract An aptamer-functionalized stir bar sorptive extraction (SBSE) coating is described for the first time devoted to selective isolation and preconcentration of an allergenic food protein, concavanalin A (Con A), followed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) determination. For this purpose, the polytetrafluoroethylene surface of commercial magnetic stir bars was properly modified and vinylized to immobilize a thiol-modified aptamer against Con A via straightforward “thiol-ene” click chemistry. The aptamer-functionalized stir bar was employed as SBSE sorbent to isolate Con A, and several parameters that can affect the extraction efficiency were investigated. Under the optimized conditions, Con A was extracted and desorbed during 30 and 45 min, respectively, at 25 °C and 600 rpm. The SBSE MALDI-TOF-MS method provided limits of detection of 0.5 μg mL −1 for Con A. Furthermore, the SBSE coating was highly selective to Con A compared to other lectins. The developed method was successfully applied to the determination of low levels of Con A in several food matrices (i.e., white beans as well as chickpea, lentils, and wheat flours). Recoveries ranged from 81 to 97% with relative standard deviations below 7%. The aptamer-based stir bars presented suitable physical and chemical long-term stability (1 month) and a reusability of 10 and 5 extraction cycles with standards and food extracts, respectively. The developed aptamer-affinity extraction devices open up the possibility of developing novel highly selective SBSE coatings for the extraction of proteins and peptides from complex samples. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-023-05795-y. Keywords: Affinity sorbent, Allergenic protein, Modified PTFE magnet, MS analysis, Sample preparation

CITATION EXPORT

María Vergara-Barberán, Mónica Catalá-Icardo, Ernesto F Simó-Alfonso et al.. Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS. Mikrochimica Acta. (2023). https://doi.org/10.1007/s00604-023-05795-y

AUTHORS

5 · 3 CENTRES

María Vergara-Barberán, Mónica Catalá-Icardo, Ernesto F Simó-Alfonso, Fernando Benavente, José Manuel Herrero-Martínez

1Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA•UB), University of Barcelona, C/Martí i Franquès 1-11, 08028 Barcelona, Spain
2Department of Analytical Chemistry, University of Valencia, C/Doctor Moliner 50E, 46100 Burjassot, Valencia Spain
3Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Campus de Gandia, Universitat Politècnica de València, C/Paranimf 1, 46730 Grau de Gandia, Valencia Spain