Association of HOTAIR gene rs920778 (C > T) and rs4759314 (A > G) polymorphism with breast cancer in Egyptian women

Molecular Biology Reports · 5 authors, 5 centres

AI SUMMARY

FIDELITY 100%

POPULATION100 Egyptian women with breast cancer and 100 unrelated healthy Egyptian female controls

INTERVENTIONGenotyping of HOTAIR gene polymorphisms rs4759314 (A > G) and rs920778 (C > T)

COMPARISONHealthy controls versus breast cancer patients

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

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TL;DR

This case-control study of 100 Egyptian breast cancer patients and 100 healthy controls found that the HOTAIR gene rs4759314 (A > G) polymorphism was associated with a significantly increased risk of breast cancer, while the rs920778 (C > T) polymorphism showed no significant association.

Full summary

931 CHARS

This study evaluated the association between two HOTAIR gene polymorphisms (rs4759314 and rs920778) and breast cancer risk in 100 Egyptian patients and 100 controls. DNA was genotyped using T-ARMS-PCR and SNP-RFLP techniques. The rs4759314 A>G variant showed a statistically significant increase in breast cancer risk under dominant (p=0.013), co-dominant (p=0.006), and overdominant (p=0.002) genetic models. In contrast, the rs920778 C>T polymorphism was not significantly associated with breast cancer. A significant association was found between ER/PR positivity with HER2 negativity and the AA genotype of rs4759314 compared to the AG genotype. No significant associations were found between either SNP and clinical stage or hormonal features. The authors conclude that rs4759314 may be a breast cancer risk factor in this population, but recommend larger studies to confirm these findings and assess HOTAIR expression levels.

PICO

PPOPULATION

100 Egyptian women with breast cancer and 100 unrelated healthy Egyptian female controls

IINTERVENTION

Genotyping of HOTAIR gene polymorphisms rs4759314 (A > G) and rs920778 (C > T)

OOUTCOME

Association of genotypes with breast cancer risk and clinicopathological features

STUDY TYPE

case-control

SPECIALTY

oncology

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10635973

Association of HOTAIR gene rs920778 (C > T) and rs4759314 (A > G) polymorphism with breast cancer in Egyptian women

Molecular Biology Reports

READ THE FULL PAPER

ABSTRACT

Background Hox transcript antisense RNA ( HOTAIR ) is considered an oncogene associated with the initiation and progression of many malignancies. Previous studies have examined the connection between HOTAIR SNPs rs4759314 and rs920778 for breast cancer (BC), getting variable results in multiple ethnicities. Therefore, this study was designed to evaluate the connection between these two SNPs and disease vulnerability, clinic-laboratory, and hormonal parameters, featuring status associations with the BC risk in an Egyptian woman sample. Methods and results In this case-control study, DNA was taken from the blood of 100 BC patients and 100 unrelated healthy Egyptian females. The characterization of rs4759314 was genotyped using the T-ARMS-PCR method and rs920778 using the SNP-RFLP technique for all participants. The frequency of the rs4759314 A > G variation revealed a statistically significant increase in BC risk with dominant (p = 0.013, OR = 1.592, 95% Cl = 1.105–2.293), co-dominant (p = 0.006, OR = 2.314, 95%Cl = 1.278–4.191) and overdominant (p = 0.002, OR = 2.571, 95% Cl = 1.430–4.624) genetic models. On the other hand, the rs920778 C > T polymorphism was not significantly associated with BC. ER/PR positivity with HER2 negativity was significantly associated with the AA genotype compared to the AG genotype. Otherwise, no significant associations between the two SNPs and clinical stage or hormonal features could be found. In conclusion, the rs4759314 A > G SNP in the HOTAIR gene is strongly associated with BC, which might warrant its determination among affected families for prevention and early treatment.

CITATION EXPORT

Nahla Anber, Mohammad M. Tarabay, Rehab Elmougy et al.. Association of HOTAIR gene rs920778 (C > T) and rs4759314 (A > G) polymorphism with breast cancer in Egyptian women. Molecular Biology Reports. (2023). https://doi.org/10.1007/s11033-023-08725-6

AUTHORS

5 · 5 CENTRES

Nahla Anber, Mohammad M. Tarabay, Rehab Elmougy, Marwa Ahmed Abdel-Dayem, Ehab Yones Elbendary

1https://ror.org/01k8vtd75grid.10251.370000 0001 0342 6662Emergency Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt
2Health Insurance Organization (HIO), Mansoura, Mansoura, 35511 Egypt
3https://ror.org/01k8vtd75grid.10251.370000 0001 0342 6662Biochemistry Division, Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt
4Pharmacology and Toxicology Department, Faculty of Pharmacy, Horus University, New Damietta City, Egypt
5https://ror.org/02bjnq803grid.411831.e0000 0004 0398 1027Clinical Nutrition Department, College of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia