Bacterial Microbiota of Ostreobium , the Coral-Isolated Chlorophyte Ectosymbiont, at Contrasted Salinities

Microorganisms · 6 authors, 2 centres

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Seven core bacterial taxa, including potential intracellular symbionts, were found to be persistent across both salinities and algal genotypes.

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Seven core bacterial taxa, including potential intracellular symbionts, were found to be persistent across both salinities and algal genotypes. A key limitation is that the study used propagated laboratory strains, which may not fully represent wild populations. The findings suggest that microbiota plasticity contributes to Ostreobium's high ecological tolerance, potentially supporting its host's metabolism and adaptation to environmental stressors.

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preclinical research

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ORIGINAL PAPER

PMC10223477

Bacterial Microbiota of Ostreobium , the Coral-Isolated Chlorophyte Ectosymbiont, at Contrasted Salinities

Microorganisms

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ABSTRACT

Abstract Microscopic filaments of the siphonous green algae Ostreobium (Ulvophyceae, Bryopsidales) colonize and dissolve the calcium carbonate skeletons of coral colonies in reefs of contrasted salinities. Here, we analyzed their bacterial community’s composition and plasticity in response to salinity. Multiple cultures of Pocillopora coral-isolated Ostreobium strains from two distinct rbc L lineages representative of IndoPacific environmental phylotypes were pre-acclimatized (>9 months) to three ecologically relevant reef salinities: 32.9, 35.1, and 40.2 psu. Bacterial phylotypes were visualized for the first time at filament scale by CARD-FISH in algal tissue sections, within siphons, at their surface or in their mucilage. Ostreobium -associated microbiota, characterized by bacterial 16S rDNA metabarcoding of cultured thalli and their corresponding supernatants, were structured by host genotype ( Ostreobium strain lineage), with dominant Kiloniellaceae or Rhodospirillaceae (Alphaproteobacteria, Rhodospirillales) depending on Ostreobium lineage, and shifted Rhizobiales’ abundances in response to the salinity increase. A small core microbiota composed of seven ASVs (~1.5% of thalli ASVs, 19–36% cumulated proportions) was persistent across three salinities in both genotypes, with putative intracellular Amoebophilaceae and Rickettsiales_AB1, as well as Hyphomonadaceae and Rhodospirillaceae also detected within environmental ( Ostreobium -colonized) Pocillopora coral skeletons. This novel knowledge on the taxonomic diversity of Ostreobium bacteria paves the way to functional interaction studies within the coral holobiont. Keywords: Ulvophyceae, bacterial communities, microbiota, endosymbionts, salinity, 16S rDNA, metabarcoding, CARD-FISH

CITATION EXPORT

Anaïs Massé, Juliette Detang, Charlotte Duval et al.. Bacterial Microbiota of Ostreobium, the Coral-Isolated Chlorophyte Ectosymbiont, at Contrasted Salinities. Microorganisms. (2023). https://doi.org/10.3390/microorganisms11051318

AUTHORS

6 · 2 CENTRES

Anaïs Massé, Juliette Detang, Charlotte Duval, Sébastien Duperron, Anthony C Woo, Isabelle Domart-Coulon

1Molécules de Communication et Adaptation des Microorganismes (MCAM), Muséum National d’Histoire Naturelle (MNHN), CNRS (UMR7245), CP54, 63 Rue Buffon, 75005 Paris, France
2Pôle Analyse de Données UAR 2700 2AD, Muséum National d’Histoire Naturelle (MNHN), 43 Rue Cuvier, 75005 Paris, France