Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

PLOS ONE · 14 authors, 9 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

This preclinical study used a bioassay-guided method to isolate berberine from Berberis lycium root extract.

Full summary

694 CHARS

This preclinical study used a bioassay-guided method to isolate berberine from Berberis lycium root extract. The compound was confirmed via TLC, HPLC, FTIR, and NMR. Its neuroprotective effects were evaluated in an aluminium chloride-induced rat model of Alzheimer's disease using behavioural tests (EPM, MWM), HPLC for neurotransmitters, western blot for β-secretase, and molecular docking. Results indicated that berberine treatment significantly improved memory patterns, enhanced dopamine and serotonin levels, increased antioxidant enzyme activity, and decreased β-secretase expression in brain tissue. Docking studies suggested a strong binding affinity between berberine and β-secretase.

STUDY TYPE

other

SPECIALTY

neurology

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10619822

Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

PLOS ONE

READ THE FULL PAPER

ABSTRACT

Abstract Objective Berberis lycium is an indigenous plant of Pakistan that is known for its medicinal properties. In the current study, we investigated the anti-Alzheimer’s effect of berberine isolated from Berberis lycium . Methods Root extract of B . lycium was subjected to acetylcholinesterase inhibition assay and column chromatography for bioassays guided isolation of a compound. The neuroprotective and memory improving effects of isolated compound were evaluated by aluminium chloride induced Alzheimer’s disease rat model, elevated plus maze (EPM) and Morris water maze (MWM) tests., Levels of dopamine and serotonin in rats brains were determined using HPLC. Moreover, western blot and docking were performed to determine interaction between berberine and β-secretase. Results During fractionation, ethyl acetate and methanol (3:7) fraction was collected from solvent mixture of ethyl acetate and methanol. This fraction showed the highest anti-acetylcholinesterase activity and was alkaloid positive. The results of TLC and HPLC analysis indicated the presence of the isolated compound as berberine. Additionally, the confirmation of isolated compound as berberine was carried out using FTIR and NMR analysis. In vivo EPM and MWM tests showed improved memory patterns after berberine treatment in Alzheimer’s disease model. The levels of dopamine, serotonin and activity of antioxidant enzymes were significantly (p<0.05) enhanced in brain tissue homogenates of berberine treated group. This was supported by decreased expression of β-secretase in berberine treated rat brain homogenates and good binding affinity of berberine with β-secretase in docking studies. Binding energies for interaction of β-secretase with berberine and drug Rivastigmine is -7.0 kcal/mol and -5.8 kcal/mol respectively representing the strong interactions. The results of docked complex of secretase with berberine and Rivastigmine was carried out using Gromacs which showed significant stability of complex in terms of RMSD and radius of gyration. Overall, the study presents berberine as a potential drug against Alzheimer’s disease by providing evidence of its effects in improving memory, neurotransmitter levels and reducing β-secretase expression in the Alzheimer’s disease model.

CITATION EXPORT

Hammad Ismail, Dania Khalid, Durdana Waseem et al.. Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking. PLOS ONE. (2023). https://doi.org/10.1371/journal.pone.0286349

AUTHORS

14 · 9 CENTRES

Hammad Ismail, Dania Khalid, Durdana Waseem, Muhammad Umar Ijaz, Erum Dilshad, Ihsan-ul Haq, Muhammad Zeeshan Bhatti, Sadaf Anwaar, Madiha Ahmed, Samreen Saleem, Milkyas Endale, Milkyas Endale, Milkyas Endale, Milkyas Endale

1Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan
2Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan
3Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan
4Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad, Pakistan
5Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan
6Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan
7Department of Biological Sciences, International Islamic University, Islamabad, Pakistan
8Department of Nutrition and Lifestyle Medicine, Health Services Academy, Islamabad, Pakistan
9Adama Science and Technology University, ETHIOPIA