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TL;DR
This study cloned the full-length quail METTL3 coding sequence and used overexpression experiments in QM7 myoblast cells to show that METTL3 promotes both myoblast proliferation and differentiation.
Veterinary Sciences · 8 authors, 4 centres
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This study cloned the full-length quail METTL3 coding sequence and used overexpression experiments in QM7 myoblast cells to show that METTL3 promotes both myoblast proliferation and differentiation.
The authors cloned the full-length quail METTL3 coding sequence (1683 bp, encoding 560 amino acids) via 3' RACE and characterized it through phylogenetic and homology analysis. In the QM7 quail myoblast cell line, Cell Counting Kit-8 assays and flow cytometry demonstrated that METTL3 promotes myoblast proliferation. Overexpression of METTL3 significantly increased expression of the differentiation markers MYOG, MYOD1, and MEF2C, further indicating that METTL3 promotes myoblast differentiation. Spatiotemporal expression analysis in quail leg muscle showed METTL3 expression gradually decreased from embryonic day E9 as differentiation progressed. These findings are limited to in vitro experiments in a single quail myoblast cell line, and the molecular mechanisms underlying METTL3-mediated regulation of QM7 cell differentiation require further exploration.