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other·ophthalmology, cornea, preclinical research, biomedical research·PMC10262482

Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits

BMC Ophthalmology · 8 authors, 4 centres

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TL;DR

The study involved inoculating rabbit corneas with one of the organisms, with control groups left untreated.

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The study involved inoculating rabbit corneas with one of the organisms, with control groups left untreated. CXL was administered one week after inoculation and abscess formation. For Fusarium solani, CXL treatment resulted in a statistically significant reduction in colony-forming units (CFU) over four weeks, with no growth by week four. For Pseudomonas aeruginosa, there was a significant CFU reduction at one week post-CXL, but regrowth occurred in all samples subsequently.

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other

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ophthalmology

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ORIGINAL PAPER

PMC10262482

Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits

BMC Ophthalmology

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ABSTRACT

Abstract Background and purpose Collagen cross-linking (CXL) has evolved as an essential therapeutic approach for corneal infections, allowing for rapidly eliminating the infecting microorganism while reducing inflammation. This study aims to evaluate the efficacy of CXL as a monotherapy for managing infectious keratitis caused by Fusarium solani and Pseudomonas aeruginosa. Materials and methods Forty-eight white New Zealand rabbits weighing approximately 1.5–2 KG were included. The cornea of one eye of each rabbit was inoculated with either Fusarium solani or Pseudomonas aeruginosa . Group A served as a control and was subdivided into two subgroups, A1 and A2; each subgroup consisted of 8 eyes and was injected with either Fusarium solani or Pseudomonas aeruginosa, respectively. Group B (16 eyes) was inoculated with Fusarium solani , while group C (16 eyes) were inoculated with Pseudomonas aeruginosa . All animals in Group B and C received CXL treatment one week after inoculation of the organisms and after corneal abscess formation was confirmed. At the same time, animals in Group A were left untreated. Results There was a statistically significant reduction in the number of colony-forming units (CFU) in Group B following CXL. No growth existed in any samples at the end of the 4th week. There was a statistically significant difference in the number of CFU between group B and the control group ( p < 0.001). In group C, there was a statistically significant reduction in the CFU at the end of the first week after CXL. However, there was regrowth in all samples afterward. All 16 models in Group C showed uncountable and extensive growth during the subsequent follow-ups. There was no statistically significant difference between the number of CFU in Group C and the control group. Histopathology showed lesser corneal melting in CXL-treated Pseudomonas aeruginosa . Conclusions Collagen cross-linking is promising monotherapy and alternative treatment in managing infective keratitis caused by Fusarium solani but is less effective in Pseudomonas aeruginosa as monotherapy. Keywords: Collagen cross-linking, Microbial keratitis, Fusarium solani , Pseudomonas aeruginosa

CITATION EXPORT

Zeinab A Saad, Hazem Elnashar, Sahar Negm et al.. Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits. BMC Ophthalmology. (2023). https://doi.org/10.1186/s12886-023-03007-y

AUTHORS

8 · 4 CENTRES

Zeinab A Saad, Hazem Elnashar, Sahar Negm, Hala A Elsayed, Mohamed Gaber Abdallah, Tamer M M Abuamara, Wagih M Abd-Elhay, Hanan M Elghonemy

1Memorial Institute of Ophthalmic Research, Giza, 12511 Egypt
2Research Institute of Ophthalmology, Giza, Egypt
3Faculty of Medicine, Department of Medical Biochemistry, Al-Azhar University, Cairo, Egypt
4Faculty of Medicine, Histology Department, Al-Azhar University, Cairo, Egypt