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other·biomedical research, preclinical research, antioxidants, nutrition, micronutrients·PMC10056211

Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts

Molecules · 5 authors, 4 centres

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TL;DR

This study phytochemically profiled different parts of the chia plant and tested their antioxidant and antimicrobial activities in vitro. Leaf extracts showed the strongest antibacterial activity against Gram-positive bacteria, while sprout extracts had the highest contribution of rosmarinic acid to antioxidant activity.

Full summary

161 CHARS

All extracts exhibited antibacterial and antifungal activity, with leaf extracts demonstrating the strongest bactericidal effects against Gram-positive bacteria.

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other

SPECIALTY

biomedical research

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AI pipeline

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100% · A

ORIGINAL PAPER

PMC10056211

Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts

Molecules

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ABSTRACT

The purpose of this study was to evaluate the phytochemical profiles of the seeds, sprouts, leaves, flowers, roots and herb of Salvia hispanica and to demonstrate their significant contribution to antioxidant and antimicrobial activities. Applied methods were: HPLC-DAD coupled with post-column derivatization with ABTS reagent, untargeted metabolomics performed by LC-Q-Orbitrap HRMS, and two-fold micro-dilution broth method, which involved suspending a solution of tested compounds dissolved in DMSO in Mueller–Hinton broth for bacteria or Mueller–Hinton broth with 2% glucose for fungi. Metabolomic profiling using LC-Q-Orbitrap HRMS used in this study yielded the identification and preliminary characterization of one hundred fifteen compounds. The dominant class of compounds was terpenoids (31 compounds), followed by flavonoids (21 compounds), phenolic acids and derivatives (19 compounds), organic acids (16 compounds) and others (fatty acids, sugars and unidentified compounds). The organic and phenolic acids were the most abundant classes in terms of total peak area, with distribution depending on the plant raw materials obtained from S. hispanica. The main compound among this class for all types of extracts was rosmarinic acid which was proven to be the most abundant for antioxidant potential. All tested extracts exhibited considerable antibacterial and antifungal activity. The strongest bioactivity was found in leaf extracts, which presented bactericidal activity against Gram-positive bacteria ( S. aureus , S. epidermidis , M. luteus and E. faecalis ). The work represents the first compendium of knowledge comparing different S. hispanica plant raw materials in terms of the profile of biologically active metabolites and their contribution to antioxidant, antimicrobial and antifungal activity.

CITATION EXPORT

Sara Motyka, Barbara Kusznierewicz, Halina Ekiert et al.. Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts. Molecules. (2023). https://doi.org/10.3390/molecules28062728

AUTHORS

5 · 4 CENTRES

Sara Motyka, Barbara Kusznierewicz, Halina Ekiert, Izabela Korona-Głowniak, Agnieszka Szopa

1Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland; [email protected] (S.M.); [email protected] (H.E.)
2Doctoral School of Medical and Health Sciences, Collegium Medicum, Jagiellonian University, 16 Św. Łazarza St., 30-530 Kraków, Poland
3Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk, Poland
4Department of Pharmaceutical Microbiology, Medical Univeristy of Lublin, Chodźki 1 St., 20-093 Lublin, Poland; [email protected]

EDITOR / REVIEWER

Rudolf Bauer, Jelena S. Katanic Stankovic