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other·research methods, preclinical research, biomedical research, ophthalmology·PMC10910492

Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide

Biotechniques · 11 authors, 8 centres

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The study compared four analytical techniques for quantifying hydrogen sulfide (H2S) in simulated physiological aqueous solutions: a modified colorimetric ethylene blue method, a reverse-phase HPLC method, a voltammetric ion-selective electrode, and an amperometric microelectrode. The colorimetric method was linear from 1.5 to 100 μM with accuracy >94%. The HPLC method also showed linearity (R² >0.99) and specificity. Electrochemical methods demonstrated higher sensitivity, quantifying H2S in nanomole and picomole ranges with faster response times. The study concluded that method selection should depend on required sensitivity, response time, and cost, with electrochemical techniques best for high-sensitivity needs and colorimetric/HPLC methods appropriate for higher concentration ranges.

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research methods

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ORIGINAL PAPER

PMC10910492

Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide

Biotechniques

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ABSTRACT

Background: Hydrogen sulfide (H 2 S), an endogenous gasotransmitter, has potential applications in several conditions. However, its quantification in simulated physiological solutions is a major challenge due to its gaseous nature and other physicochemical properties. Aim: This study was designed to compare four commonly used H 2 S detection and quantification methods in aqueous solutions. Methods: The four techniques compared were one colorimetric, one chromatographic and two electrochemical methods. Results: Colorimetric and chromatographic methods quantified H 2 S in millimolar and micromole ranges, respectively. The electrochemical methods quantified H 2 S in the nanomole and picomole ranges and were less time-consuming. Conclusion: The H 2 S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.

CITATION EXPORT

Susmit Mhatre, Anjali Rai, Hatim Ali et al.. Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide. Biotechniques. (2023). https://doi.org/10.2144/btn-2023-0075

AUTHORS

11 · 8 CENTRES

Susmit Mhatre, Anjali Rai, Hatim Ali, Akash Patil, Neetu Singh, Richa Verma, John Auden, Cole Chandler, Alekha Dash, Catherine Opere, Somnath Singh

1Department of Pharmacy Sciences, School of Pharmacy & Health Professions, Creighton University, Omaha, NE 68178, USA
2Hikma Pharmaceuticals, Bedford, OH 44146, USA
3Large Molecule Product Development, Johnson & Johnson Greater Philadelphia, Philadelphia, PA 19355, USA
4Department of Molecular Pharmaceutics, University of Utah, Salt Lake City, UT 84112, USA
5Cardiovascular & Metabolic Research Unit, York University, Toronto, ON, M3J 1P3, Canada
6Department of Biomolecular Sciences, Laurentian University, Sudbury, ON, P3E 2C6, Canada
7Department of Chemistry & Biochemistry, College of Arts & Sciences, Creighton University, Omaha, NE 68178, USA
8Department of Biology, College of Arts & Sciences, Creighton University, Omaha, NE 68178, USA