Correlation between Bacterial Cell Density and Abundance of Antibiotic Resistance on Milking Machine Surfaces Assessed by Cultivation and Direct qPCR Methods

Microbial Ecology · 5 authors, 2 centres

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The authors conclude that dense microbial communities on food-associated surfaces may serve as hotspots for antibiotic resistance gene distribution.

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The authors conclude that dense microbial communities on food-associated surfaces may serve as hotspots for antibiotic resistance gene distribution.

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other

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preclinical research

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ORIGINAL PAPER

PMC10497690

Correlation between Bacterial Cell Density and Abundance of Antibiotic Resistance on Milking Machine Surfaces Assessed by Cultivation and Direct qPCR Methods

Microbial Ecology

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ABSTRACT

The relative abundance of antibiotic-resistant bacteria and antibiotic-resistance genes was surveyed for different parts of a milking machine. A cultivation approach based on swab samples showed a highly diverse microbiota, harboring resistances against cloxacillin, ampicillin, penicillin, and tetracycline. This approach demonstrated a substantial cloxacillin resistance of numerous taxa within milking machine microbiota coming along with regular use of cloxacillin for dry-off therapy of dairy cows. For the less abundant tetracycline-resistant bacteria we found a positive correlation between microbial cell density and relative abundance of tetracycline-resistant microorganisms (R 2 = 0.73). This indicated an accelerated dispersion of resistant cells for sampling locations with high cell density. However, the direct quantification of the tetM gene from the swap samples by qPCR showed the reverse relation to bacterial density if normalized against the abundance of 16S rRNA genes (R 2 = 0.88). The abundance of 16S rRNA genes was analyzed by qPCR combined with a propidium monoazide treatment, which eliminates 16S rRNA gene signals in negative controls. Supplementary Information The online version contains supplementary material available at 10.1007/s00248-023-02225-7.

CITATION EXPORT

Mareike Weber, Bettina Göpfert, Sina von Wezyk et al.. Correlation between Bacterial Cell Density and Abundance of Antibiotic Resistance on Milking Machine Surfaces Assessed by Cultivation and Direct qPCR Methods. Microbial Ecology. (2023). https://doi.org/10.1007/s00248-023-02225-7

AUTHORS

5 · 2 CENTRES

Mareike Weber, Bettina Göpfert, Sina von Wezyk, Michael Savin-Hoffmeyer, André Lipski

1grid.10388.320000 0001 2240 3300Institute of Nutrition and Food Sciences, Department of Food Microbiology and Hygiene, University of Bonn, Friedrich-Hirzebruch-Allee 7, 53115 Bonn, Germany
2grid.15090.3d0000 0000 8786 803XInstitute for Hygiene and Public Health, University Hospital Bonn, Venusberg-Campus 1, 53127 Bonn, Germany