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TL;DR
It concludes that while the foundational methods remain valid, improvements such as serum-free, chemically defined media could make the technique more accessible.
Pathogens · 1 author, 2 centres
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It concludes that while the foundational methods remain valid, improvements such as serum-free, chemically defined media could make the technique more accessible.
It describes the foundational work by Trager and Jensen and Haynes et al., the use of RPMI 1640 medium and human serum, and the significant advance of using Albumax as a serum substitute. The review discusses how cultivation has enabled vast biological, biochemical, immunological, and pharmacological research. It identifies limitations of current methods, including the need for human erythrocytes and serum, and outlines future directions such as developing serum-free, chemically defined media.