International Journal of Molecular Sciences · 12 authors, 1 centre
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C57BL/6J mice received either singular 5 Gy or fractional 10 Gy total body irradiation, and 60 days later showed decreased spatial memory, reduced activity, and bone density loss. The fractional 10 Gy group failed to recover cognitively or motorically, while the singular 5 Gy group showed time-dependent recovery, suggesting fractional 10 Gy TBI as a stable multi-organ injury model.
C57BL/6J mice were exposed to singular 5 Gy or fractional 10 Gy total body irradiation (109 cGy/min), and delayed effects were assessed 60 days post-irradiation through behavioral testing, histopathology (HE, Nissl, Trap staining), micro-CT scanning, RNA-Seq, and Western blot. Both irradiation groups exhibited decreased spatial memory and activity, reduced bone density, and elevated osteoclast counts. The fractional 10 Gy group showed significantly lower BMD, BS, and BV/TV compared with controls and did not recover, while the singular 5 Gy group demonstrated time-dependent cognitive and motor recovery. Molecular analyses indicated that bone loss was associated with down-regulation of RUNX2, sclerostin, and beta-catenin, and brain injury was linked to down-regulation of BDNF and Tau. A common mechanism involving p53-mediated oxidative stress and apoptosis through DNA damage was implicated.
