SEARCHOTHERantioxidants, preclinical research

other·antioxidants, preclinical research, biomedical research, glaucoma, neurology·PMC10058309

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate ( Punica granatum )—A Chemical Profiling by LC-MS/MS

Life · 4 authors, 4 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

This preclinical study used in vitro assays to evaluate the antioxidant capacities and enzyme inhibitory effects of Zivzik pomegranate extracts.

Full summary

540 CHARS

This study investigated the biological properties of Zivzik pomegranate (Punica granatum) using in vitro biochemical assays. Researchers analyzed ethanol and water extracts from the peel and seed, as well as juice, for antioxidant capacity via ABTS and DPPH radical scavenging and reducing power assays. The ethanol peel extract showed strong antioxidant activity, with IC50 values of 5.9 and 16.1 μg/mL for ABTS and DPPH scavenging, respectively. LC-MS/MS profiling identified 30 phenolic compounds, with ellagic acid as the most abundant.

STUDY TYPE

other

SPECIALTY

antioxidants

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10058309

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate ( Punica granatum )—A Chemical Profiling by LC-MS/MS

Life

READ THE FULL PAPER

ABSTRACT

Zivzik pomegranate ( Punica granatum ) has recently sparked considerable interest due to its nutritional and antioxidant properties. To evaluate the antioxidant capacities of P. granatum juice, ethanol (EEZP), and water (WEZP) extracts from peel and seed, the antioxidant methods of 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS •+ ) scavenging, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH • ) scavenging, Fe 3+ -2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing, Fe 3+ reducing, and Cu 2+ reducing methods were used. The antioxidant capacities of samples were compared with the most commonly used synthetic antioxidants, i.e., BHA, BHT, α-tocopherol, and Trolox. In terms of setting an example, the IC 50 values of EEZP for ABTS •+ and DPPH • scavenging activities were found to be lower than standards, at 5.9 and 16.1 μg/mL, respectively. The phenolic and flavonoid contents in EEZP peel were 59.7 mg GAE/g and 88.0 mg QE/g, respectively. Inhibition of α-glycosidase, α-amylase, acetylcholinesterase, and human carbonic anhydrase II (hCA II) enzymes was also investigated. EEZP demonstrated IC 50 values of 7.3 μg/mL against α-glycosidase, 317.7 μg/mL against α-amylase, 19.7 μg/mL against acetylcholinesterase (AChE), and 106.3 μg/mL against CA II enzymes. A total of 53 phenolic compounds were scanned, and 30 compounds were determined using LC-MS/MS. E. coli and S. aureus bacteria were resistant to all four antibiotics used as standards in hospitals.

CITATION EXPORT

Hasan Karagecili, Ebubekir İzol, Ekrem Kirecci et al.. Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)—A Chemical Profiling by LC-MS/MS. Life. (2023). https://doi.org/10.3390/life13030735

AUTHORS

4 · 4 CENTRES

Hasan Karagecili, Ebubekir İzol, Ekrem Kirecci, İlhami Gulcin

1Department of Nursing, Faculty of Health Sciences, Siirt University, 56100 Siirt, Turkey
2Bee and Natural Products R & D and P & D Application and Research Center, Bingöl University, 12000 Bingol, Turkey
3Department of Chemistry, Faculty of Science, Ataturk University, 25240 Erzurum, Turkey
4Department of Basic Medical Sciences, Faculty of Medicine, Microbiology, Kahramanmaraş Sütçü İmam University, 46050 Kahramanmaras, Turkey

EDITOR / REVIEWER

Balazs Barna, Jianfeng Xu