Aquaculture Nutrition · 7 authors, 2 centres
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Common carp were starved for 14 days and then supplemented with BCAAs via gavage for 14 days, followed by transcriptome and small RNA sequencing of skeletal muscle. BCAA supplementation after starvation was associated with downregulation of ubiquitin-proteasome and autophagy pathway genes and upregulation of muscle growth-associated genes, suggesting BCAAs may alleviate muscle protein catabolism under starvation stress.
Common carp (Cyprinus carpio) were subjected to 14 days of starvation followed by 14 days of oral gavage with BCAAs, and skeletal muscle was analyzed via transcriptome and small RNA sequencing. A total of 43,414 known and 1,112 novel genes were identified, along with 142 known and 654 novel miRNAs. Among these, 2,146 differentially expressed genes and 84 differentially expressed miRNAs were detected. KEGG pathway enrichment indicated involvement of the proteasome, phagosome, autophagy, and ubiquitin-dependent protein catabolic processes. BCAA supplementation was associated with downregulation of ubiquitin-proteasome pathway genes (uba3, ube1l2, sae1, cdc53) and autophagy-related genes (atg5, map1lc3c, vmp1, pik3c3), alongside upregulation of muscle growth-associated genes (myh9s, myl9, mylk, fosb, mapk12a).
