SEARCHOTHERpediatric ophthalmology, biomedical research

other·pediatric ophthalmology, biomedical research, preclinical research·PMC10401786

Direct and residual antimicrobial effect of 2% chlorhexidine gel, double antibiotic paste and chitosan- chlorhexidine nanoparticles as intracanal medicaments against Enterococcus faecalis and Candida albicans in primary molars: an in-vitro study

BMC Oral Health · 3 authors, 1 centre

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TL;DR

This in-vitro study tested the antimicrobial effect of three intracanal medicaments on infected primary molar roots. The chitosan-chlorhexidine nanoparticles showed the highest direct and residual effect against both Candida albicans and Enterococcus faecalis, with performance comparable to chlorhexidine and double antibiotic paste respectively.

Full summary

737 CHARS

This in-vitro study evaluated the direct and residual antimicrobial effects of 2% chlorhexidine gel, 500 mg/ml double antibiotic paste, and chitosan-chlorhexidine nanoparticles on 63 infected primary molar roots against Enterococcus faecalis and Candida albicans. Microbiological samples were taken after 3 and 7 days of medicament placement, and 7 days after removal. Chitosan-chlorhexidine nanoparticles had the highest anticandidal effect, comparable to chlorhexidine, and the highest effect against E. faecalis at 3 days, comparable to double antibiotic paste. All medicaments showed similar effect against E. faecalis after 7 days. Chitosan-chlorhexidine nanoparticles showed the highest residual effect against both microorganisms.

STUDY TYPE

other

SPECIALTY

pediatric ophthalmology

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100% · A

ORIGINAL PAPER

PMC10401786

Direct and residual antimicrobial effect of 2% chlorhexidine gel, double antibiotic paste and chitosan- chlorhexidine nanoparticles as intracanal medicaments against Enterococcus faecalis and Candida albicans in primary molars: an in-vitro study

BMC Oral Health

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ABSTRACT

Background Thorough disinfection of root canals in primary molars may be complicated by the complex root canal morphology. This in-vitro study aimed to compare direct and residual antimicrobial effect of 2% chlorhexidine (CHX) gel, 500 mg/ml double antibiotic paste (DAP) and chitosan-chlorhexidine nanoparticles (CS-CHX NPs) as intracanal medicaments against Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in primary molars. Methods Mesial roots of 63 mandibular second primary molars were infected with E. faecalis and C. albicans . Teeth were divided into 9 groups: Ia: (CS-CHX NPs), IIa: (CHX), IIIa: (DAP), IVa: chitosan nanoparticles (CSNPs) in which medicaments were placed for 3 days, groups Ib: (CS-CHX NPs), IIb: CHX, IIIb: (DAP), IVb: (CSNPs) in which medicaments were placed for 7 days, and Group V (control): teeth were infected, irrigated with saline, and sampled 3- and 7-days post-infection. Microbiological samples were obtained after infection, 3, and 7 days after medicament placement and 7 days after medicament removal for both time points. One-way ANOVA, Tukey’s post hoc test and paired t-test were used at p < 0.05. Results CS-CHX NPs had the highest anticandidal effect which was comparable to CHX and significantly higher than other medicaments (p < 0.001) at both time points. CS-CHX NPs had the highest effect against E. faecalis which was comparable to DAP and significantly higher than other medicaments (p < 0.001) at 3 days. All medicaments showed similar effect against E. faecalis after 7 days. The 7-days placement significantly increased the antimicrobial effect against both micro-organisms in all groups, except CS-CHX NPs which showed an insignificant increase. CS-CHX NPs showed the highest residual effect against both micro-organisms that increased with 7-days placement. Conclusion CSNPs and CHX combination showed a synergistic effect against both micro-organisms. CS-CHX NPs displayed a higher effect at a shorter period compared to other medicaments, yet its residual effect was higher with 7-days placement.

CITATION EXPORT

Mariem Wassel, Mohamed Radwan, Reham Elghazawy. Direct and residual antimicrobial effect of 2% chlorhexidine gel, double antibiotic paste and chitosan- chlorhexidine nanoparticles as intracanal medicaments against Enterococcus faecalis and Candida albicans in primary molars: an in-vitro study. BMC Oral Health. (2023). https://doi.org/10.1186/s12903-023-02862-x

AUTHORS

3 · 1 CENTRES

Mariem Wassel, Mohamed Radwan, Reham Elghazawy

grid.7269.a0000 0004 0621 1570Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Ain Shams University Cairo, Organization of African Unity St.-Abbasia-Cairo, 11566 Cairo, Egypt