Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks

BMC Oral Health · 11 authors, 4 centres

AI SUMMARY

FIDELITY 100%

POPULATIONEighteen male New Zealand white rabbits undergoing bilateral sinus augmentation

INTERVENTIONHorizontal platelet-rich fibrin (H-PRF) bone block combined with deproteinized bovine bone mineral (DBBM)

COMPARISONDBBM alone

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

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TL;DR

Eighteen male New Zealand white rabbits underwent bilateral maxillary sinus augmentation and were divided into two groups: DBBM alone and H-PRF plus DBBM.

Full summary

916 CHARS

Eighteen male New Zealand white rabbits underwent bilateral maxillary sinus augmentation and were divided into two groups: DBBM alone and H-PRF plus DBBM. In vitro Transwell assays evaluated migration of osteoblasts and THP-1 macrophages using conditioned media. The H-PRF bone block group showed higher immune cell infiltration at 3 and 7 days, with significantly greater new blood vessel formation and early osteoclast development at 14 days compared to DBBM alone. In vitro experiments confirmed that H-PRF conditioned media promoted migration of both osteoblasts and macrophages. Minor soft tissue edema was observed with no adverse events such as Schneiderian membrane perforation or wound dehiscence. Limitations include the absence of immunofluorescence staining to identify specific immune cell types and inability to distinguish cells derived from PRF itself versus those recruited from neighboring tissues.

PICO

PPOPULATION

Eighteen male New Zealand white rabbits undergoing bilateral sinus augmentation

IINTERVENTION

Horizontal platelet-rich fibrin (H-PRF) bone block combined with deproteinized bovine bone mineral (DBBM)

OOUTCOME

Histological assessment of inflammatory cells, new blood vessel formation, osteoclast bone turnover, and in vitro migration of osteoblasts and macrophages

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10463479

Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks

BMC Oral Health

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ABSTRACT

Background The effects of horizontal platelet-rich fibrin (H-PRF) bone block on the healing and immune response during sinus augmentation have not been fully investigated histologically at early time points. Methods Eighteenth male New Zealand white rabbits underwent bilateral sinus augmentation and were divided into two groups: deproteinized bovine bone mineral (DBBM) alone and H-PRF‚Äâ+‚ÄâDBBM (H-PRF bone block) group. Maxilla samples were collected at 3, 7 and 14 days post sinus augmentation procedures and analyzed using histological staining for the number of inflammatory cells, new blood vessels and evidence for early osteoclast bone turnover/remodeling. Furthermore, the effects of H-PRF bone blocks on the migration of osteoblasts and THP-1 macrophages were evaluated using a Transwell assay in vitro. Results A higher number of immune cells were found in the H-PRF bone block group at 3 and 7 days post-surgery when compared to the DBBM alone group,most notably in the regions close to the mucosal lining and bone plates. Furthermore, a significantly greater number of new blood vessel formations and early signs of osteoclast development were found in the H-PRF bone block group at 14 days. The in vitro transwell assay further confirmed that culture medium from H-PRF bone block markedly promote the migration of osteoblasts and THP-1 macrophages. Conclusions The findings from this study have shown that H-PRF bone block is capable of increasing early immune cell infiltration leading to the acceleration of neovascularization and speeding the process of bone metabolism in vivo following maxillary sinus grafting with DBBM.

CITATION EXPORT

Shimin Yu, Yinping Tian BD, Yan Wei BD et al.. Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks. BMC Oral Health. (2023). https://doi.org/10.1186/s12903-023-03228-z

AUTHORS

11 · 4 CENTRES

Shimin Yu, Yinping Tian BD, Yan Wei BD, Mengge Feng BD, Sensen Li BMed, Guoyong Tong BMed, Zhouqing Yu BMed, Richard J. Miron, Yufeng Zhang, Zaibo Yang, Yulan Wang

1https://ror.org/033vjfk17grid.49470.3e0000 0001 2331 6153State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079 China
2https://ror.org/01s12ye51grid.507043.50000 0005 1089 2345Department of Stomatology, The Central Hospital of Enshi Tujia and Miao Autonomous Perfecture, Enshi, 445000 China
3https://ror.org/02k7v4d05grid.5734.50000 0001 0726 5157Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
4https://ror.org/033vjfk17grid.49470.3e0000 0001 2331 6153Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071 China