SEARCHRCTgeriatrics, nutrition

RCT·geriatrics, nutrition, clinical nutrition, internal medicine, preclinical research·PMC10421766

Effect of 1-year daily protein supplementation and physical exercise on muscle protein synthesis rate and muscle metabolome in healthy older Danes: a randomized controlled trial

European Journal of Nutrition · 8 authors, 5 centres

AI SUMMARY

FIDELITY 100%

POPULATIONHealthy older Danes (>65 years, 29 females, 37 males)

INTERVENTION12-month daily protein supplementation (collagen or whey) with or without home-based or center-based resistance training

COMPARISONCarbohydrate (isocaloric) supplementation

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

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TL;DR

The study found no effect of the interventions on basal or postprandial muscle protein synthesis rates or the muscle metabolome.

Full summary

594 CHARS

Using stable isotope tracer infusions, researchers measured basal and postprandial MPS in response to a protein-glucose drink. The main finding was that neither protein supplementation alone nor combined with exercise significantly altered basal or postprandial MPS rates or the muscle metabolome compared to a carbohydrate control. The study also explored sex differences, finding no significant difference in basal MPS between males and females. The study provides novel reference data on the human muscle metabolome but suggests caution in extrapolating results to long-term recommendations.

PICO

PPOPULATION

Healthy older Danes (>65 years, 29 females, 37 males)

IINTERVENTION

12-month daily protein supplementation (collagen or whey) with or without home-based or center-based resistance training

OOUTCOME

Basal and postprandial muscle protein synthesis rate (MPS) and skeletal muscle metabolome

STUDY TYPE

RCT

SPECIALTY

geriatrics

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10421766

Effect of 1-year daily protein supplementation and physical exercise on muscle protein synthesis rate and muscle metabolome in healthy older Danes: a randomized controlled trial

European Journal of Nutrition

READ THE FULL PAPER

ABSTRACT

Abstract Background The skeletal muscle mass decreases with age and the responsiveness of aging muscles’ protein synthesis rate (MPS) to protein intake seems to deteriorate. Objective This study investigated the impact of 12 months of protein supplementation with or without physical exercise training on the basal and postprandial MPS and the skeletal muscle metabolome of healthy older Danes (> 65 years, 29 females/37 males). Methods Subjects were randomized to follow one of five intervention groups: (1) carbohydrate, (2) collagen protein, (3) whey protein, (4) home-based light resistance training with whey protein, and (5) center-based heavy-load resistance training with whey protein. Before and after the intervention, a tracer infusion trial was conducted to measure basal and postprandial MPS in response to intake of a cocktail consisting of 20 g whey hydrolysate + 10 g glucose. In addition, the skeletal muscle metabolome was measured using gas chromatography–mass spectrometry (GC–MS) at basal state and 4 h after the intake of the cocktail. Results One year of daily protein or carbohydrate supplementation did not alter the basal and protein-stimulated postprandial muscle protein synthesis rate or the muscle metabolome of healthy older Danes. Basal MPS (%/h) at baseline for all subjects were 0.0034 ± 0,011 (mean ± SD). In contrast to previous studies, no difference was observed in basal MPS between males and females ( p = 0.75). With the developed untargeted GC–MS methodology, it was possible to detect and tentatively annotate > 70 metabolites from the human skeletal muscle samples. Conclusion One year of protein supplementation in comparison to an isocaloric-control supplement seems to affect neither the MPS at basal or postprandial state nor the skeletal muscle metabolome. Clinical trial registry Number: NCT02115698 , clinicaltrials.gov/ct2/show/ NCT02115698 . Supplementary Information The online version contains supplementary material available at 10.1007/s00394-023-03182-0. Keywords: Muscle metabolome, Protein synthesis, Aging, Protein supplementation, Training, Sarcopenia, Healthy older adults, Healthy aging

CITATION EXPORT

Jacob Bülow, Bekzod Khakimov, Søren Reitelseder et al.. Effect of 1-year daily protein supplementation and physical exercise on muscle protein synthesis rate and muscle metabolome in healthy older Danes: a randomized controlled trial. European Journal of Nutrition. (2023). https://doi.org/10.1007/s00394-023-03182-0

AUTHORS

8 · 5 CENTRES

Jacob Bülow, Bekzod Khakimov, Søren Reitelseder, Rasmus Bechshøft, Mikkel Jensen, Gerrit van Hall, Søren Balling Engelsen, Lars Holm

1Department of Orthopedic Surgery M, Institute of Sports Medicine Copenhagen M81, Bispebjerg Hospital, Building 8, Level 1, Nielsine Nielsens Vej 11, 2400 Copenhagen NV, Denmark
2Department of Food Sciences, University of Copenhagen, Copenhagen, Denmark
3Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
4Clinical Metabolomics Core Facility, Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
5School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK