Escherichia albertii as a Potential Enteropathogen in the Light of Epidemiological and Genomic Studies

Genes · 8 authors, 4 centres

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TL;DR

The study isolated 42 E. albertii strains from bird droppings, food, water, and human fecal samples across multiple years, finding that human stool samples yielded no isolates while biochemical profiling and whole-genome sequencing revealed high genetic diversity and several virulence and resistance genes.

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The study screened 497 bird droppings, 1154 human fecal samples, 212 food samples, and 92 water samples, isolating 42 E. albertii strains, with none recovered from human stool, indicating distribution mainly restricted to birds and the environment. Diagnostic PCR proved useful because 38.1% of isolates had atypical biochemical profiles, leading to misidentification as E. coli or H. alvei by commercial systems; a majority (80%) of biochemical profiles were matched with other species at an unacceptable probability. Antibiotic susceptibility testing of 42 isolates showed 33.3% resistant to one antibiotic and 16.7% resistant to two, with ampicillin resistance most common (45.0%) and no ESBL production. Whole-genome sequencing of 25 strains revealed chromosomes ranging from 4,573,338 to 5,141,010 bp and 1–8 plasmids per strain, with 90.5% of strains showing distinct PFGE pulsotypes and 85.7% distinct MLST sequence types.

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other

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infectious disease

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AI pipeline

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ORIGINAL PAPER

PMC10379040

Escherichia albertii as a Potential Enteropathogen in the Light of Epidemiological and Genomic Studies

Genes

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ABSTRACT

Abstract Escherichia albertii is a new enteropathogen of humans and animals. The aim of the study was to assess the prevalence and pathogenicity of E. albertii strains isolated in northeastern Poland using epidemiological and genomic studies. In 2015–2018, a total of 1154 fecal samples from children and adults, 497 bird droppings, 212 food samples, 92 water samples, and 500 lactose-negative E. coli strains were tested. A total of 42 E. albertii strains were isolated. The PCR method was suitable for their rapid identification. In total, 33.3% of E. albertii isolates were resistant to one antibiotic, and 16.7% to two. Isolates were sensitive to cefepime, imipenem, levofloxacin, gentamicin, trimethoprim/sulfamethoxazole, and did not produce ESBL β-lactamases. High genetic variability of E. albertii has been demonstrated. In the PFGE method, 90.5% of the strains had distinct pulsotypes. In MLST typing, 85.7% of strains were assigned distinct sequence types (STs), of which 64% were novel ST types. Cytolethal distending toxin (CDT) and Paa toxin genes were found in 100% of E. albertii isolates. Genes encoding toxins, IbeA, CdtB type 2, Tsh and Shiga (Stx2f), were found in 26.2%, 9.7%, 1.7%, and 0.4% of E. albertii isolates, respectively. The chromosome size of the tested strains ranged from 4,573,338 to 5,141,010 bp (average 4,784,003 bp), and at least one plasmid was present in all strains. The study contributes to a more accurate assessment of the genetic diversity of E. albertii and the potential threat it poses to public health. Keywords: Escherichia albertii , enteropathogen, diarrhea, Shiga toxin, antibiotic susceptibility, intimin, pathogenicity island LEE, genome, pangenome

CITATION EXPORT

Katarzyna Leszczyńska, Izabela Święcicka, Tamara Daniluk et al.. Escherichia albertii as a Potential Enteropathogen in the Light of Epidemiological and Genomic Studies. Genes. (2023). https://doi.org/10.3390/genes14071384

AUTHORS

8 · 4 CENTRES

Katarzyna Leszczyńska, Izabela Święcicka, Tamara Daniluk, Dariusz Lebensztejn, Sylwia Chmielewska-Deptuła, Dorota Leszczyńska, Jan Gawor, Małgorzata Kliber

1Department of Medical Microbiology and Nanobiomedical Enginnering, Medical University of Bialystok, ul. Mickiewicza 2C, 15-222 Bialystok, Poland
2Department of Microbiology and Biotechnology, University of Bialystok, ul. Ciołkowskiego 1J, 15-245 Białystok, Poland
3Department of Pediatrics, Gastroenterology, Hepatology, Nutrition, Allergology and Pulmonology, Medical University of Bialystok, ul. Waszyngtona 17, 15-274 Bialystok, Poland
4DNA Sequecing and Synthesis Facility, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warszawa, Poland; [email protected]