Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway

Foods · 8 authors, 2 centres

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TL;DR

In a rat model of irritable bowel syndrome with constipation (IBS-C), the ethanol extract of Mao Jian Green Tea (MJGT_EE) increased fecal water content, promoted gastric emptying and intestinal propulsion, and reduced visceral hypersensitivity.

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Irritable bowel syndrome with constipation (IBS-C) is a functional gastrointestinal disorder. This preclinical study investigated the therapeutic effects of the ethanol extract of Mao Jian Green Tea (MJGT_EE) in a rat model of IBS-C constructed via maternal separation and ice water stimulation. The study first confirmed successful model construction based on fecal water content (FWC) and colorectal distension (CRD) threshold. Treatment with MJGT_EE significantly increased FWC and the smallest CRD volume, indicating improvement in constipation and visceral hypersensitivity. It also promoted gastric emptying and small intestinal propulsion. Limitations include the use of a specific animal model that may not fully represent human IBS-C pathogenesis and the need for further clinical validation.

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other

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preclinical research

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ORIGINAL PAPER

PMC10000491

Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway

Foods

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ABSTRACT

In a previous study, we demonstrated that the hydro extract of Mao Jian Green Tea (MJGT) promotes gastrointestinal motility. In this study, the effect of MJGT ethanol extract (MJGT_EE) in treating irritable bowel syndrome with constipation (IBS-C) in a rat model constructed via maternal separation combined with an ice water stimulation was investigated. First, a successful model construction was confirmed through the determination of the fecal water content (FWC) and the smallest colorectal distension (CRD) volume. Then, the overall regulatory effects of MJGT_EE on the gastrointestinal tract were preliminarily evaluated through gastric emptying and small intestinal propulsion tests. Our findings indicated that MJGT_EE significantly increased FWC ( p < 0.01) and the smallest CRD volume ( p < 0.05) and promoted gastric emptying and small intestinal propulsion ( p < 0.01). Furthermore, mechanistically, MJGT_EE reduced intestinal sensitivity by regulating the expression of proteins related to the serotonin (5-hydroxytryptamine; 5-HT) pathway. More specifically, it decreased tryptophan hydroxylase (TPH) expression ( p < 0.05) and increased serotonin transporter (SERT) expression ( p < 0.05), thereby decreasing 5-HT secretion ( p < 0.01), activating the calmodulin (CaM)/myosin light chain kinase (MLCK) pathway, and increasing 5-HT 4 receptor (5-HT 4 R) expression ( p < 0.05). Moreover, MJGT_EE enhanced the diversity of gut microbiota, increased the proportion of beneficial bacteria, and regulated the number of 5-HT-related bacteria. Flavonoids may play the role of being active ingredients in MJGT_EE. These findings suggest that MJGT_EE could serve as a potential therapeutic pathway for IBS-C.

CITATION EXPORT

Lei Wu, Liming Gao, Xiang Jin et al.. Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway. Foods. (2023). https://doi.org/10.3390/foods12051101

AUTHORS

8 · 2 CENTRES

Lei Wu, Liming Gao, Xiang Jin, Zhikang Chen, Xutong Qiao, Xiting Cui, Jianhua Gao, Liwei Zhang

1Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
2Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China

EDITOR / REVIEWER

Maria Eduardo-Figueira, Rosa Direito