Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in citrus relatives with a specific focus on flowering

Molecular Horticulture · 9 authors, 3 centres

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TL;DR

This study identified and characterized SPL genes across citrus-related species. It found that overexpressing specific citrus SPL genes (FhSPL5, FhSPL9, FhSPL11) in transgenic Arabidopsis led to precocious flowering by upregulating the endogenous FT gene.

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The study systematically identified members of the SPL gene family in 15 citrus-related species and analyzed their expression in adult and young tissues. Promoter analysis indicated SPL genes are involved in diverse developmental processes. To test function, the citrus orthologs FhSPL5, FhSPL9, and FhSPL11 were independently overexpressed in Arabidopsis. This ectopic expression resulted in enhanced expression of the endogenous flowering gene FT and caused significantly precocious flowering in the transgenic lines.

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other

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preclinical research

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ORIGINAL PAPER

PMC10515035

Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in citrus relatives with a specific focus on flowering

Molecular Horticulture

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ABSTRACT

Phase transition and floral induction in citrus requires several years of juvenility after germination. Such a long period of juvenility has been a major hindrance to its genetic improvement program. Studies have shown that miR156 along with its downstream genes SQUAMOSA PROMOTER BINDING PROTEINS (SBP) and SBP-LIKE (SPL) mediate the phase transition and floral induction process in plants. Our current study has systematically analyzed SPL s in 15 different citrus-related species, systematically annotated them based on their close homology to their respective Arabidopsis orthologs, and confirmed the functional attributes of the selected members in floral precocity. The majority of the species harbored 15 SPL s. Their cis -element assessment suggested the involvement of the SPL s in diverse developmental and physiological processes in response to different biotic and abiotic cues. Among all, SPL5 , SPL9 , and SPL11 stood out as consistently differentially expressed SPL s in the adult and young tissues of different citrus-related species. Independent overexpression of their F. hindsii orthologs ( FhSPL5 , FhSPL9 , and FhSPL11 ) brought an enhanced expression of endogenous FLOWERING LOCUS T leading to the significantly precocious flowering in transgenic Arabidopsis lines. Future study of the genes in the citrus plant itself is expected to conclude the assessments made in the current study. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s43897-023-00061-4.

CITATION EXPORT

Yawei Li, Shuting Wang, Prakash Babu Adhikari et al.. Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in citrus relatives with a specific focus on flowering. Molecular Horticulture. (2023). https://doi.org/10.1186/s43897-023-00061-4

AUTHORS

9 · 3 CENTRES

Yawei Li, Shuting Wang, Prakash Babu Adhikari, Bing Liu, Shengjun Liu, Yue Huang, Gang Hu, Michitaka Notaguchi, Qiang Xu

1grid.35155.370000 0004 1790 4137National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, 430000 China
2grid.27476.300000 0001 0943 978XBioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601 Japan
3Hubei Hongshan Laboratory, Wuhan, 430070 China