SEARCHOTHERpreclinical research, gastroenterology

other·preclinical research, gastroenterology, infectious disease, immunology, biomedical research·PMC10813405

Exploring PLGA-OH-CATH30 Microspheres for Oral Therapy of Escherichia coli -Induced Enteritis

Biomolecules · 7 authors, 1 centre

AI SUMMARY

FIDELITY 100%

POPULATIONMice with E. coli-induced enteritis

INTERVENTIONOral PLGA-OH-CATH30 microspheres (POC group)

COMPARISONGentamicin sulfate (GS group), direct oral OH-CATH30 peptide without encapsulation (OC group), untreated infected model group, and healthy control group

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

The microsphere treatment also shifted the gut microbiota toward beneficial bacterial populations.

Full summary

486 CHARS

The POC treatment reduced pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), increased the anti-inflammatory cytokine IL-10, and mitigated damage to the intestinal mucosal barrier, with efficacy on par with gentamicin sulfate. Gut microbiota analysis showed that POC treatment decreased harmful bacteria and increased beneficial genera including Phocaeicola vulgatus and Blautia, whereas direct oral OH-CATH30 (OC group) did not significantly improve symptoms compared to the model group.

PICO

PPOPULATION

Mice with E. coli-induced enteritis

IINTERVENTION

Oral PLGA-OH-CATH30 microspheres (POC group)

OOUTCOME

Inflammatory cytokine levels, intestinal barrier integrity, gut microbiota composition, and clinical symptoms

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10813405

Exploring PLGA-OH-CATH30 Microspheres for Oral Therapy of Escherichia coli -Induced Enteritis

Biomolecules

READ THE FULL PAPER

ABSTRACT

Abstract Antibiotic therapy effectively addresses Escherichia coli -induced enteric diseases, but its excessive utilization results in microbial imbalance and heightened resistance. This study evaluates the therapeutic efficacy of orally administered poly (lactic-co-glycolic acid) (PLGA)-loaded antimicrobial peptide OH-CATH30 microspheres in murine bacterial enteritis. Mice were categorized into the healthy control group (CG), untreated model group (MG), OH-CATH30 treatment group (OC), PLGA-OH-CATH30 treatment group (POC), and gentamicin sulfate treatment group (GS). Except for the control group, all other experimental groups underwent Escherichia coli -induced enteritis, followed by a 5-day treatment period. The evaluation encompassed clinical symptoms, intestinal morphology, blood parameters, inflammatory response, and gut microbiota. PLGA-OH-CATH30 microspheres significantly alleviated weight loss and intestinal damage while also reducing the infection-induced increase in spleen index. Furthermore, these microspheres normalized white blood cell count and neutrophil ratio, suppressed inflammatory factors (IL-1β, IL-6, and TNF-α), and elevated the anti-inflammatory factor IL-10. Analysis of 16S rRNA sequencing results demonstrated that microsphere treatment increased the abundance of beneficial bacteria, including Phocaeicola vulgatus , in the intestinal tract while concurrently decreasing the abundance of pathogenic bacteria, such as Escherichia . In conclusion, PLGA-OH-CATH30 microspheres have the potential to ameliorate intestinal damage and modulate the intestinal microbiota, making them a promising alternative to antibiotics for treating enteric diseases induced by Escherichia coli . Keywords: Escherichia coli , enteritis, PLGA microspheres, antimicrobial peptide, inflammatory, gut microbiome

CITATION EXPORT

Xiaoqian Jiao, Bin Liu, Xufeng Dong et al.. Exploring PLGA-OH-CATH30 Microspheres for Oral Therapy of Escherichia coli-Induced Enteritis. Biomolecules. (2024). https://doi.org/10.3390/biom14010086

AUTHORS

7 · 1 CENTRES

Xiaoqian Jiao, Bin Liu, Xufeng Dong, Shubai Wang, Xiulei Cai, Hongliang Zhang, Zhihua Qin

1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China; [email protected] (X.J.); [email protected] (B.L.); [email protected] (X.D.); [email protected] (S.W.); [email protected] (X.C.); [email protected] (H.Z.)