Genome-wide identification and characterization analysis of RWP-RK family genes reveal their role in flowering time of Chrysanthemum lavandulifolium

BMC Plant Biology · 6 authors, 4 centres

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TL;DR

This study identified 101 RWP-RK genes in Chrysanthemum lavandulifolium and found that optimal nitrate conditions advanced flowering time by up-regulating specific floral integration genes and cryptochromes.

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The study conducted a genome-wide identification and characterization of RWP-RK family genes in the Compositae plant Chrysanthemum lavandulifolium, identifying 101 genes, including 38 NIN-like proteins (NLPs) and 31 RWP-RK domain proteins (RKDs). Expression analysis via qRT-PCR revealed that 7 ClNLPs genes in leaves were up-regulated at the floral transition stage. Low nitrate conditions negatively regulated 10 ClNLPs genes, while optimal nitrate conditions up-regulated 3 of them. Flowering time was advanced under optimal nitrate conditions, which also up-regulated the expression of photoperiod pathway genes (ClCRYs, ClPHYC) and floral integration genes (ClGI, ClCOLs, ClFT, ClFLC, ClSOC1). The expression levels of specific ClCRY genes induced by optimal nitrate in the vegetative stage matched those induced by short-day in the reproductive stage. The findings suggest ClNLPs may act in the photoperiodic pathway under optimal nitrate to regulate flowering.

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other

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preclinical research

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ORIGINAL PAPER

PMC10105424

Genome-wide identification and characterization analysis of RWP-RK family genes reveal their role in flowering time of Chrysanthemum lavandulifolium

BMC Plant Biology

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ABSTRACT

Abstract Background RWP-RKs are plant specific transcription factors, which are widely distributed in plants in the form of polygenic families and play key role in nitrogen absorption and utilization, and are crucial to plant growth and development. However, the genome-wide identification and function of RWP-RK in Compositae plants are widely unknown. Results In this study, 101 RWP-RKs in Chrysanthemum lavandulifolium were identified and tandem repeat was an important way for the expansion of RWP-RKs in Compositae species. 101 RWP-RKs contain 38 NIN-like proteins (NLPs) and 31 RWP- RK domain proteins (RKDs), as well as 32 specific expansion members. qRT-PCR results showed that 7 ClNLPs in leaves were up-regulated at the floral transition stage, 10 ClNLPs were negatively regulated by low nitrate conditions, and 3 of them were up-regulated by optimal nitrate conditions. In addition, the flowering time of Chrysanthemum lavandulifolium was advanced under optimal nitrate conditions, the expression level of Cryptochromes ( ClCRYs ), phytochrome C ( ClPHYC ) and the floral integration genes GIGANTEA ( ClGI ), CONSTANS-LIKE ( ClCOL1 , ClCOL4 , ClCOL5 ), FLOWERING LOCUS T ( ClFT ), FLOWERING LOCUS C ( ClFLC ), SUPPRESSOR OF OVER-EXPRESSION OF CONSTANS 1 ( ClSOC1 ) also were up-regulated. The expression level of ClCRY1a , ClCRY1c , ClCRY2a and ClCRY2c in the vegetative growth stage induced by optimal nitrate reached the expression level induced by short-day in the reproductive growth stage, which supplemented the induction effect of short-day on the transcription level of floral-related genes in advance. Conclusions It was speculated that ClNLPs may act on the photoperiodic pathway under optimal nitrate environment, and ultimately regulate the flowering time by up-regulating the transcription level of ClCRYs . These results provide new perspective for exploring the mechanism of nitrate/nitrogen affecting flowering in higher plants. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-023-04201-2. Keywords: Nitrogen, Nitrate, RWP-RK, Photoperiod pathway, Flowering time

CITATION EXPORT

Qiuling Zhang, Junzhuo Li, Xiaohui Wen et al.. Genome-wide identification and characterization analysis of RWP-RK family genes reveal their role in flowering time of Chrysanthemum lavandulifolium. BMC Plant Biology. (2023). https://doi.org/10.1186/s12870-023-04201-2

AUTHORS

6 · 4 CENTRES

Qiuling Zhang, Junzhuo Li, Xiaohui Wen, Chengyan Deng, Xiuzhen Yang, Silan Dai

1Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Education Ministry, School of Landscape Architecture, Beijing Forestry University, Beijing, 100083 China
2Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058 China
3Linyi University, Shandong, 276000 China
4School of Landscape Architecture, Beijing Forestry University, Beijing, 100083 China