Animals : an Open Access Journal from MDPI · 3 authors, 3 centres
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Primary head kidney cells from rainbow trout were treated with two synthetic ghrelin isoforms and gene expression was measured over time by RT-qPCR. The two isoforms exerted overlapping and divergent modulatory effects on immune-related and antimicrobial peptide genes at different time points.
This in vitro study treated primary rainbow trout head kidney (HKD) cells with two synthetic ghrelin isoforms — full-length ghrelin and its three-amino-acid truncated variant desVRQ-ghrelin — at time points of 0, 2, 4, and 24 hours. Gene expression of immune-related and antimicrobial peptide genes was measured by RT-qPCR. The two isoforms differentially modulated suppressin expression, and both affected cathelicidin genes cath1 and cath2, with changes described as potentially subtle in magnitude or timing. The authors conclude the two ghrelin analogs may activate unique pathways, eliciting distinct immune responses.
