Hybrid De Novo Whole-Genome Assembly, Annotation, and Identification of Secondary Metabolite Gene Clusters in the Ex-Type Strain of Chrysosporium keratinophilum

Journal of Fungi · 5 authors, 1 centre

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The analysis revealed the strain's genetic capability to produce secondary metabolites, suggesting a significant biosynthetic potential for compounds of biotechnological interest.

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The analysis revealed the strain's genetic capability to produce secondary metabolites, suggesting a significant biosynthetic potential for compounds of biotechnological interest. The authors note that future in vitro studies are required to confirm the actual production of these molecules. The work is a foundational genomic resource for further investigation within the Onygenales order.

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biomedical research

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ORIGINAL PAPER

PMC10145314

Hybrid De Novo Whole-Genome Assembly, Annotation, and Identification of Secondary Metabolite Gene Clusters in the Ex-Type Strain of Chrysosporium keratinophilum

Journal of Fungi

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ABSTRACT

Abstract Chrysosporium is a polyphyletic genus belonging (mostly) to different families of the order Onygenales (Eurotiomycetes, Ascomycota). Certain species, such as Chrysosporium keratinophilum , are pathogenic for animals, including humans, but are also a source of proteolytic enzymes (mainly keratinases) potentially useful in bioremediation. However, only a few studies have been published regarding bioactive compounds, of which the production is mostly unpredictable due to the absence of high-quality genomic sequences. During the development of our study, the genome of the ex-type strain of Chrysosporium keratinophilum , CBS 104.66, was sequenced and assembled using a hybrid method. The results showed a high-quality genome of 25.4 Mbp in size spread across 25 contigs, with an N50 of 2.0 Mb, 34,824 coding sequences, 8002 protein sequences, 166 tRNAs, and 24 rRNAs. The functional annotation of the predicted proteins was performed using InterProScan, and the KEGG pathway mapping using BlastKOALA. The results identified a total of 3529 protein families and 856 superfamilies, which were classified into six levels and 23 KEGG categories. Subsequently, using DIAMOND, we identified 83 pathogen–host interactions (PHI) and 421 carbohydrate-active enzymes (CAZymes). Finally, the analysis using AntiSMASH showed that this strain has a total of 27 biosynthesis gene clusters (BGCs), suggesting that it has a great potential to produce a wide variety of secondary metabolites. This genomic information provides new knowledge that allows for a deeper understanding of the biology of C. keratinophilum , and offers valuable new information for further investigations of the Chrysosporium species and the order Onygenales. Keywords: ANI, Ascomycota, biosynthetic pathways, Chrysosporium keratinophilum , genome, Onygenales

CITATION EXPORT

Alan Omar Granados-Casas, Angie Paola Sastoque, Alberto Miguel Stchigel et al.. Hybrid De Novo Whole-Genome Assembly, Annotation, and Identification of Secondary Metabolite Gene Clusters in the Ex-Type Strain of Chrysosporium keratinophilum. Journal of Fungi. (2023). https://doi.org/10.3390/jof9040389

AUTHORS

5 · 1 CENTRES

Alan Omar Granados-Casas, Angie Paola Sastoque, Alberto Miguel Stchigel, Ana Fernández-Bravo, José Francisco Cano-Lira

1Mycology Unit, Medical School, Universitat Rovira i Virgili, C/Sant Llorenç 21, 43201 Reus, Spain