SEARCHOTHERophthalmology, ocular immunology

other·ophthalmology, ocular immunology, preclinical research, biomedical research, research methods·PMC10470687

Identification of the key mechanisms of action of Si-Ni-San in uveitis using bioinformatics and network pharmacology

Medicine · 7 authors, 5 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

This computational study used network pharmacology and molecular docking to predict that Si-Ni-San (SNS) may treat uveitis through multi-component, multi-target synergistic action involving the bioactive compounds kaempferol, luteolin, quercetin, and naringin acting on core targets JUN, RELA, and MAPK1.

Full summary

662 CHARS

This in silico study used network pharmacology and molecular docking to investigate mechanisms of Si-Ni-San (SNS) in treating uveitis. Active components and predicted targets were mined through TCMSP, DrugBank, GEO, and GeneCards databases. A protein-protein interaction network of SNS-uveitis intersection genes yielded 170 nodes and 414 edges. Molecular docking of core targets (JUN, RELA/MAPK1) with four bioactive compounds showed favorable binding energies ranging from -7.4 to -8.8 kcal/mol. These findings provide reference-level predictions suggesting SNS may regulate immune and inflammatory pathways in uveitis, but experimental confirmation is needed.

STUDY TYPE

other

SPECIALTY

ophthalmology

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10470687

Identification of the key mechanisms of action of Si-Ni-San in uveitis using bioinformatics and network pharmacology

Medicine

READ THE FULL PAPER

ABSTRACT

Background: Uveitis is an eye disease with a high rate of blindness, whose pathogenesis is not completely understood. Si-Ni-San (SNS) has been used as a traditional medicine to treat uveitis in China. However, its mechanism of action remains unclear. This study explored the potential mechanisms of SNS in the treatment of uveitis through network pharmacology and bioinformatics. Methods: Using R language and Perl software, the active components and predicted targets of SNS, as well as the related gene targets of uveitis, were mined through the Traditional Chinese Medicine Systems Pharmacology, Therapeutic Target, Gene Expression Omnibus, GeneCards, and DrugBank databases. The network diagram of active components and intersection targets was constructed using Cytoscape software and the String database. The CytoNCA plug-in was used to conduct topological analysis on the network diagram and screen out the core compounds and key targets. The genes were analyzed for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment. Chemoffice, Pymol, AutoDock, and Vina were used to analyze the molecular docking of key targets and core compounds of diseases through the PubChem database. Results: JUN, RELA, and MAPK may play important roles in the treatment of uveitis by SNS. Kyoto encyclopedia of genes and genomes pathway enrichment analysis showed that core genes were mainly concentrated in MAPK, toll-like receptor, tumor necrosis factor, and nucleotide oligomerization domain-like receptor signaling pathways. In addition, molecular docking results showed that the bioactive compounds (kaempferol, luteolin, naringin, and quercetin) exhibited good binding ability to JUN, RELA, and MAPK. Conclusion: Based on these findings, SNS exhibits multi-component and multi-target synergistic action in the treatment of uveitis, and its mechanism may be related to anti-inflammatory and immune regulation.

CITATION EXPORT

Dandan Zhang, Liu Hong, Rui Su Zhang et al.. Identification of the key mechanisms of action of Si-Ni-San in uveitis using bioinformatics and network pharmacology. Medicine. (2023). https://doi.org/10.1097/MD.0000000000034615

AUTHORS

7 · 5 CENTRES

Dandan Zhang, Liu Hong, Rui Su Zhang, Qian Zhang, Jing Yao, Jiadi Wang, Ning Zhang

a Dalian Women and Children’s Medical Group, Dalian, China
b The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Ha Er Bin Shi, China
c Heilongjiang University of Chinese Medicine, Harbin, China
d The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China
e Banan Hospital of Chongqing Medical University, Chongqing, China.