Microorganisms · 8 authors, 5 centres
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This study used metagenomic sequencing on 18 RCDI patients to identify functional changes in gut microbiota after fecal microbial transplant. It found 27 statistically significant KEGG orthology groups post-FMT, particularly in pathways for iron homeostasis, glycerol metabolism, and arginine regulation.
This exploratory longitudinal cohort study analyzed stool shotgun metagenomic sequencing data from 18 RCDI patients who underwent successful FMT. Taxonomic and functional profiling assessed changes in KEGG orthology (KO) groups at baseline (0 weeks) and 1, 4, and 12 weeks post-FMT. Of 59,987 KO groups identified, 27 showed a statistically significant change. The authors hypothesize these functional shifts may contribute to FMT efficacy beyond restored microbial diversity. Limitations include the small sample size (n=18), lack of metabolomics for validation, reliance on shallow sequencing and existing databases, and the absence of failed FMT cases for comparison. The findings are hypothesis-generating and require validation through larger studies, metabolomics, and animal models.
