In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications

International Journal of Molecular Sciences · 4 authors, 4 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

It synthesizes findings from in vitro laboratory assays and in vivo implantation studies in small animal models.

Full summary

615 CHARS

It synthesizes findings from in vitro laboratory assays and in vivo implantation studies in small animal models. The central results indicate that these constructs show favorable biological performance, including maintenance of cell viability and promotion of cell growth, proliferation, and differentiation. The review concludes that while progress is promising for regenerating bone, periodontal, dentin, and pulp tissues, significant gaps remain. Limitations include the preclinical nature of the evidence and the need for more studies to address technical, ethical, and legal issues before clinical translation.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10454183

In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications

International Journal of Molecular Sciences

READ THE FULL PAPER

ABSTRACT

Abstract Three-dimensional (3D) bioprinting is a unique combination of technological advances in 3D printing and tissue engineering. It has emerged as a promising approach to address the dilemma in current dental treatments faced by clinicians in order to repair or replace injured and diseased tissues. The exploration of 3D bioprinting technology provides high reproducibility and precise control of the bioink containing the desired cells and biomaterial over the architectural and dimensional features of the scaffolds in fabricating functional tissue constructs that are specific to the patient treatment need. In recent years, the dental applications of different 3D bioprinting techniques, types of novel bioinks, and the types of cells used have been extensively explored. Most of the findings noted significant challenges compared to the non-biological 3D printing approach in constructing the bioscaffolds that mimic native tissues. Hence, this review focuses solely on the implementation of 3D bioprinting techniques and strategies based on cell-laden bioinks. It discusses the in vitro applications of 3D-bioprinted scaffolds on cell viabilities, cell functionalities, differentiation ability, and expression of the markers as well as the in vivo evaluations of the implanted bioscaffolds on the animal models for bone, periodontal, dentin, and pulp tissue regeneration. Finally, it outlines some perspectives for future developments in dental applications. Keywords: regenerative dentistry, dental tissues regeneration, three-dimensional bioprinting, cell laden, bioink, tissue engineering

CITATION EXPORT

Nurulhuda Mohd, Masfueh Razali, Mh Busra Fauzi et al.. In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications. International Journal of Molecular Sciences. (2023). https://doi.org/10.3390/ijms241612881

AUTHORS

4 · 4 CENTRES

Nurulhuda Mohd, Masfueh Razali, Mh Busra Fauzi, Noor Hayaty Abu Kasim

1Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia; [email protected]
2Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia; [email protected]
3Department of Restorative Dentistry, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur 50603, Malaysia
4Dean Office, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia