Cell & Bioscience · 4 authors, 4 centres
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This narrative review summarizes methods for predicting and detecting endogenous G-quadruplex DNA structures in living cells, discusses factors affecting their dynamics, and explores their potential as therapeutic targets, especially in cancer.
This is a narrative review summarizing current knowledge on the formation, detection, regulation, and biological roles of endogenous G-quadruplex (eG4) structures in mammalian systems. The authors describe methods for predicting potential G-quadruplex-forming sequences and detecting eG4s in cells, such as cell imaging and high-throughput sequencing. They highlight factors influencing eG4 dynamics and their effects, noting that eG4 folding is important for telomere maintenance while unfolding contributes to genomic DNA stability. The review concludes that regulating eG4 dynamics with stabilizing ligands is a confirmed effective therapy for cancer. Limitations include the need for improved resolution and sensitivity of existing research methods.
