Insulin peptides and their receptors regulate ovarian development and oviposition behavior in Diaphorina citri

Insect Science · 8 authors, 5 centres

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TL;DR

The central result was that silencing DcILP1, DcILP2, and DcIR significantly inhibited ovarian development and reduced egg laying.

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They found that gene silencing resulted in a significant decrease in the number of eggs laid and inhibited ovarian development. Further analysis using RT-qPCR showed that the expression of the vitellogenin gene DcVG, crucial for egg development, decreased significantly following interference. The results indicate that the insulin signaling pathway, activated by nutritional signals from host plant flush shoots, regulates reproduction by influencing the TOR signaling pathway and vitellogenin expression.

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other

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preclinical research

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ORIGINAL PAPER

PMC10084437

Insulin peptides and their receptors regulate ovarian development and oviposition behavior in Diaphorina citri

Insect Science

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ABSTRACT

Abstract Diaphorina citri is an important vector of Citrus Huanglongbing (HLB) disease. After feeding on young host plant shoots, the population of D. citri can increase significantly. Females also only lay eggs on young shoots. However, there are few studies on the mechanism of this phenomenon. Exogenous nutrient signals can affect the insulin signaling system of D. citri after feeding on young shoots. In this study, the expression of upstream factors DcILP1 , DcILP2 , and DcIR in the insulin signaling system of D. citri was upregulated after feeding on young shoots. After being silenced by RNA interference technology, the results showed that the number of oviposited eggs of D. citri was significantly decreased and the ovarian development was inhibited with severe vacuolation. In addition, detection using quantitative reverse transcription–polymerase chain reaction showed that the upstream regulatory gene DcRheb of the target of rapamycin (TOR) pathway and the downstream reproduction‐related DcVg gene were also significantly downregulated. These results suggest that feeding upon young shoots may upregulate the expression levels of upstream factors DcILP1 , DcILP2 , and DcIR in the insulin signaling system. The signal will be through upregulating the expression of DcRheb , an upstream gene of the TOR signaling pathway. This in turn influences yolk metabolism, which eventually causes the ovaries of female D. citri to mature and therefore initiate oviposition behavior. Keywords: Diaphorina citri , insulin‐like peptide, insulin receptor, insulin signaling, reproduction, target of rapamycin

CITATION EXPORT

Ziye Wang, Delong Tan, Feifeng Wang et al.. Insulin peptides and their receptors regulate ovarian development and oviposition behavior in Diaphorina citri. Insect Science. (2022). https://doi.org/10.1111/1744-7917.13048

AUTHORS

8 · 5 CENTRES

Ziye Wang, Delong Tan, Feifeng Wang, Shuhao Guo, Jinhua Liu, Andrew G S Cuthbertson, Baoli Qiu, Wen Sang

1 Key Laboratory of Bio‐Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Guangzhou, China
2 Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
3 Institute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou, China
4 Natural Medicine Institute of Zhejiang YangShengTang Co. LTD, Hangzhou, China
5 Independent Science Advisor, York, UK