SEARCHCOHORTophthalmology, retina

cohort·ophthalmology, retina, endocrinology, clinical trial, observational study·PMC10011241

Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study

Ophthalmology and Therapy · 5 authors, 3 centres

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TL;DR

This pilot study included 12 participants (4 healthy controls, 4 with DM, 4 with DR) undergoing intraocular surgery.

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This pilot study included 12 participants (4 healthy controls, 4 with DM, 4 with DR) undergoing intraocular surgery. The researchers collected aqueous humour and stool samples to compare the microbiomes using 16S rRNA sequencing. A key finding was a consistent reduction in the abundance of anti-inflammatory bacteria in the aqueous humour of people with DR compared to those with DM. The study's limitations include a small sample size, differences in participant ages, and its preliminary nature, requiring validation with larger studies. The findings suggest the intraocular microbiome may have specific features in DR and could be a future therapeutic target.

STUDY TYPE

cohort

SPECIALTY

ophthalmology

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AI pipeline

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100% · A

ORIGINAL PAPER

PMC10011241

Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study

Ophthalmology and Therapy

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ABSTRACT

Abstract Introduction The objective of this study was to compare the microbiome in the aqueous humour and gut of people with diabetes mellitus (DM) with and without diabetic retinopathy (DR). Methods This was a prospective controlled study. The study included 17 people undergoing intraocular surgery in their naïve eyes. Stool samples were obtained in the perioperative period; aqueous humour samples of sufficient quantity were obtained in 12 people during intraocular surgery. Dietary information was obtained using a previously validated questionnaire. The gut and aqueous humour samples were assessed for microbiome using 16S rRNA gene sequencing coupled with QIIME and R software. Results Aqueous humour was analysed in 12 people: 4 each healthy controls, people with DM, and people with DR. There were minor differences at the phyla levels, but the aqueous humour microbiomes of healthy controls, DM, and DR formed three distinct clusters on heat map analysis with discriminatory genera. This genera-level clustering was more apparent for the intraocular than the gut microbiome. In people with DM and DR, we identified genera unique to the eye or the gut. There was a consistent reduction in the abundance of anti-inflammatory bacteria in people with DR than DM. Conclusions There is a difference in intraocular and gut microbiome regardless of disease or health. Our preliminary findings indicate distinctive features of the intraocular microbiome in people with DR compared with those without it. While this distinctiveness appears more evident in aqueous humour than in the gut, it needs further confirmation with larger studies. Supplementary Information The online version contains supplementary material available at 10.1007/s40123-023-00660-w. Keywords: Microbiome, Diabetes mellitus, Diabetic retinopathy, Gut retina axis, Prevention of blindness

CITATION EXPORT

Taraprasad Das, Shalem Raj Padakandla, Sisinthy Shivaji et al.. Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study. Ophthalmology and Therapy. (2023). https://doi.org/10.1007/s40123-023-00660-w

AUTHORS

5 · 3 CENTRES

Taraprasad Das, Shalem Raj Padakandla, Sisinthy Shivaji, Rajagopalaboopathi Jayasudha, Brijesh Takkar

1Anant Bajaj Retina Institute-Srimati Kanuri Santhamma Centre for Vitreoretinal Diseases, L V Prasad Eye Institute, Hyderabad, India
2Indian Health Outcomes, Public Health, and Economics Research (IHOPE) Centre, L V Prasad Eye Institute, Hyderabad, India
3Prof. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Hyderabad, India