Analytical and Bioanalytical Chemistry · 10 authors, 2 centres
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Glycation produced pentosidine crosslinks detectable by increased TPEF signal and reduced SHG ratio, alongside elevated surface roughness but preserved collagen D-period length, in contrast to genipin crosslinking and enzymatic digestion which caused more dramatic structural disruption.
Equine pericardium samples were glycated with ribose (5–200 mM) over 10–30 days and analyzed using multimodal imaging. Raman spectroscopy with PLS-LDA multivariate analysis classified 20- and 30-day glycated pericardium with high accuracy, showing characteristic band intensity changes but no direct crosslinker marker bands. Volumetric analysis showed elastin fibers condensed superficially, with deeper layers devoid of fibers and a loss of tightly packed collagen network. Results were compared with prior genipin crosslinking, glutaraldehyde crosslinking, and collagenase digestion studies, revealing that glycation produced the slowest crosslinking reaction.
