SEARCHOTHERpreclinical research, biomedical research

other·preclinical research, biomedical research, rheumatology, dermatology, food science·PMC10415269

Investigation of the phytochemical composition, antioxidant, antibacterial, anti-osteoarthritis, and wound healing activities of selected vegetable waste

Scientific Reports · 10 authors, 11 centres

AI SUMMARY

FIDELITY 100%

POPULATIONIL-1β-stimulated mouse chondrocytes, LPS-induced Hfb4 human skin cells, and mouse wound healing model

INTERVENTIONOnion peel extract (OPE) and its nanocapsule-based hydrogel formulation

COMPARISONPotato and garlic peel extracts, celecoxib (for anti-osteoarthritis), and standard controls

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

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TL;DR

This study analyzed phytochemicals and tested biological activities of potato, onion, and garlic peel extracts. Onion peel extract showed the strongest antioxidant, anti-osteoarthritis, and wound healing effects in preclinical models.

Full summary

1,139 CHARS

This preclinical study evaluated the phytochemical composition and biological activities of potato, onion, and garlic peel extracts. A total of 47 compounds were identified via UHPLC-ESI-qTOF-MS/MS. Onion peel extract (OPE) exhibited the highest polyphenolic content and strongest antioxidant activity. In IL-1β-stimulated mouse chondrocytes, OPE significantly inhibited the overproduction of NO, MMP-13, NF-κB p65, IL-6, TNF-α, COX-2, and iNOS, suggesting anti-osteoarthritis potential. OPE showed promising antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). In an LPS-induced Hfb4 skin cell model, OPE reversed inflammatory and oxidative stress markers. In an in vivo mouse model, OPE accelerated wound healing, restoring tissue physiology mainly through activation of the AP-1 signaling pathway. A nano-formulation of OPE in a hydrogel enhanced cellular viability. Molecular docking highlighted quercetin-3,4′-O-diglucoside and other compounds as potential active entities. Limitations include the preclinical nature of the evidence, requiring further research to confirm in vivo efficacy and mechanisms.

PICO

PPOPULATION

IL-1β-stimulated mouse chondrocytes, LPS-induced Hfb4 human skin cells, and mouse wound healing model

IINTERVENTION

Onion peel extract (OPE) and its nanocapsule-based hydrogel formulation

OOUTCOME

Anti-inflammatory markers, antioxidant capacity, antibacterial activity, wound closure, and signaling pathway activation

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10415269

Investigation of the phytochemical composition, antioxidant, antibacterial, anti-osteoarthritis, and wound healing activities of selected vegetable waste

Scientific Reports

READ THE FULL PAPER

ABSTRACT

Agri-food wastes, produced following industrial food processing, are mostly discarded, leading to environmental hazards and losing the nutritional and medicinal values associated with their bioactive constituents. In this study, we performed a comprehensive analytical and biological evaluation of selected vegetable by-products (potato, onion, and garlic peels). The phytochemical analysis included UHPLC-ESI-qTOF-MS/MS in combination with molecular networking and determination of the total flavonoid and phenolic contents. Further, the antimicrobial, anti-osteoarthritis and wound healing potentials were also evaluated. In total, 47 compounds were identified, belonging to phenolic acids, flavonoids, saponins, and alkaloids as representative chemical classes. Onion peel extract (OPE) showed the higher polyphenolic contents, the promising antioxidant activity, the potential anti-osteoarthritis activity, and promising antimicrobial activity, especially against methicillin-resistant Staphylococcus aureus (MRSA). Furthermore, OPE revealed to have promising in vivo wound healing activity, restoring tissue physiology and integrity, mainly through the activation of AP-1 signaling pathway. Lastly, when OPE was loaded with nanocapsule based hydrogel, the nano-formulation revealed enhanced cellular viability. The affinities of the OPE major metabolites were evaluated against both p65 and ATF-2 targets using two different molecular docking processes revealing quercetin-3,4′- O -diglucoside, alliospiroside C, and alliospiroside D as the most promising entities with superior binding scores. These results demonstrate that vegetable by-products, particularly, those derived from onion peels can be incorporated as natural by-product for future evaluation against wounds and osteoarthritis.

CITATION EXPORT

Mohamed A. Salem, Osama G. Mohamed, Esraa M. Mosalam et al.. Investigation of the phytochemical composition, antioxidant, antibacterial, anti-osteoarthritis, and wound healing activities of selected vegetable waste. Scientific Reports. (2023). https://doi.org/10.1038/s41598-023-38591-y

AUTHORS

10 · 11 CENTRES

Mohamed A. Salem, Osama G. Mohamed, Esraa M. Mosalam, Aya Ibrahim Elberri, Hend Mohamed Abdel-Bar, Mariam Hassan, Ahmed A. Al-Karmalawy, Ashootosh Tripathi, Shahira M. Ezzat, Hend E. Abo Mansour

1https://ror.org/05sjrb944grid.411775.10000 0004 0621 4712Department of Pharmacognosy and Natural Products, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shibīn al-Kawm, 32511 Menoufia Egypt
2https://ror.org/03q21mh05grid.7776.10000 0004 0639 9286Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el Aini St., Cairo, 11562 Egypt
3https://ror.org/00jmfr291grid.214458.e0000 0004 1936 7347Natural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109 USA
4https://ror.org/05sjrb944grid.411775.10000 0004 0621 4712Biochemistry Department, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shebin El-Koum, 32511 Egypt
5https://ror.org/05sjrb944grid.411775.10000 0004 0621 4712Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, 32511 Menoufia Egypt
6https://ror.org/05p2q6194grid.449877.10000 0004 4652 351XDepartment of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt
7https://ror.org/03q21mh05grid.7776.10000 0004 0639 9286Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr el Aini st., Cairo, 11562 Egypt
8Department of Microbiology and Immunology, Faculty of Pharmacy, Galala University, New Galala City, Suez Egypt
9https://ror.org/02t055680grid.442461.10000 0004 0490 9561Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Giza, 12566 Egypt
10https://ror.org/00jmfr291grid.214458.e0000 0004 1936 7347Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 USA
11grid.442760.30000 0004 0377 4079Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, 12451 Egypt