SEARCHOTHERophthalmology, dry eye

other·ophthalmology, dry eye, preclinical research, cornea, biomedical research·PMC10454390

Isolation and Identification of a Novel Anti-Dry Eye Peptide from Tilapia Skin Peptides Based on In Silico, In Vitro, and In Vivo Approaches

International Journal of Molecular Sciences · 10 authors, 3 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

Using a combination of in silico screening, in vitro cell assays, and an in vivo mouse model, the authors isolated the peptide NGGPSGPR from tilapia skin peptides and demonstrated its anti-dry eye disease activity. NGG restored corneal epithelial cell viability, inhibited COX-2 expression in vitro, and improved multiple dry eye parameters in mice.

Full summary

686 CHARS

The authors isolated bioactive peptides from tilapia skin by enzymatic hydrolysis and Sephadex G-25 chromatography, dividing the resulting peptides into five fractions. Using LC/MS/MS, 131 peptides were identified in the active fraction TSP-II, and in silico screening predicted the octapeptide NGGPSGPR (NGG) as a candidate anti-dry eye disease peptide. In vitro, NGG restored cell viability and inhibited COX-2 protein expression in NaCl-induced human corneal epithelial cells. Statistical analysis used one-way ANOVA with Duncan's post hoc test (p < 0.05). Limitations include that only two DED models were explored and the complete molecular mechanism of NGG action remains unclear.

STUDY TYPE

other

SPECIALTY

ophthalmology

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10454390

Isolation and Identification of a Novel Anti-Dry Eye Peptide from Tilapia Skin Peptides Based on In Silico, In Vitro, and In Vivo Approaches

International Journal of Molecular Sciences

READ THE FULL PAPER

ABSTRACT

Tilapia skin is a great source of collagen. Here, we aimed to isolate and identify the peptides responsible for combating dry eye disease (DED) in tilapia skin peptides (TSP). In vitro cell DED model was used to screen anti-DED peptides from TSP via Sephadex G-25 chromatography, LC/MS/MS, and in silico methods. The anti-DED activity of the screened peptide was further verified in the mice DED model. TSP was divided into five fractions (TSP-I, TSP-II, TSP-III, TSP-IV, and TSP-V), and TSP-II exerted an effective effect for anti-DED. A total of 131 peptides were identified using LC/MS/MS in TSP-II, and NGGPSGPR (NGG) was screened as a potential anti-DED fragment in TSP-II via in silico methods. In vitro, NGG restored cell viability and inhibited the expression level of Cyclooxygenase-2 (COX-2) protein in Human corneal epithelial cells (HCECs) induced by NaCl. In vivo, NGG increased tear production, decreased tear ferning score, prevented corneal epithelial thinning, alleviated conjunctival goblet cell loss, and inhibited the apoptosis of corneal epithelial cells in DED mice. Overall, NGG, as an anti-DED peptide, was successfully identified from TSP, and it may be devoted to functional food ingredients or medicine for DED.

CITATION EXPORT

Jian Zeng, Cuixian Lin, Shilin Zhang et al.. Isolation and Identification of a Novel Anti-Dry Eye Peptide from Tilapia Skin Peptides Based on In Silico, In Vitro, and In Vivo Approaches. International Journal of Molecular Sciences. (2023). https://doi.org/10.3390/ijms241612772

AUTHORS

10 · 3 CENTRES

Jian Zeng, Cuixian Lin, Shilin Zhang, Haowen Yin, Kaishu Deng, Zhiyou Yang, Yongping Zhang, You Liu, Chuanyin Hu, Yun-Tao Zhao

1College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China
2College of Food Science and Engineering, Ocean University of China, Yu-Shan Road, Qingdao 266003, China
3Department of Biology, Guangdong Medical University, Zhanjiang 524023, China

EDITOR / REVIEWER

Bent Honoré