Life · 10 authors, 2 centres
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In this in vitro study, isorhamnetin (5–150 μM) was applied to HT-29 human colon adenocarcinoma cells for 24 h or 48 h to assess viability, superoxide production, and IL-8 biosynthesis. Isorhamnetin demonstrated dose- and time-dependent antiproliferative effects, increased superoxide generation, and reduced IL-8 production under inflammatory conditions.
HT-29 human colon adenocarcinoma cells were cultured with isorhamnetin at concentrations ranging from 5 to 150 μM for 24 h or 48 h. Viability and proliferation were assessed using MTT, metabolic activity, membrane integrity, and lysosomal activity tests. Intracellular superoxide generation was measured by NBT test, and IL-8 biosynthesis was evaluated by ELISA under basal and zymosan-stimulated inflammatory conditions. Isorhamnetin induced dose- and time-dependent growth inhibition, with significantly reduced mitochondrial activity at 100 and 150 μM after both 24 h and 48 h. Superoxide generation was significantly elevated at 100 μM after 24 h and from 40 μM after 48 h. Basal IL-8 production was effectively inhibited at 80 and 100 μM, and zymosan-stimulated IL-8 expression was altered in experimental groups with lower concentrations. This is an in vitro study without animal or human participants, and the findings may not be directly translatable to clinical outcomes.
