SEARCHOTHERophthalmology, cataract surgery

other·ophthalmology, cataract surgery, preclinical research, biomedical research·PMC10373963

MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway

Aging (Albany NY) · 7 authors, 2 centres

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TL;DR

This preclinical study used human lens epithelial cells and mouse lenses to show that microRNA-34a (MIR34A) directly targets and suppresses hexokinase 1 (HK1), leading to increased apoptosis and lens opacification through the HK1/caspase 3 pathway.

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This preclinical study investigated the role of microRNA-34a (MIR34A) and hexokinase 1 (HK1) in cataract development. Using the human lens epithelial cell line SRA01/04 and mouse lenses, the researchers demonstrated that MIR34A directly targets and suppresses HK1 expression. In vitro, upregulation of MIR34A or downregulation of HK1 inhibited cell proliferation, induced apoptosis, and increased caspase 3 activity. In a mouse model, overexpression of Mir34a or Hk1 downregulation accelerated lens opacification. The authors conclude that MIR34A modulates lens epithelial cell apoptosis and cataract formation via the HK1/caspase 3 signaling pathway.

STUDY TYPE

other

SPECIALTY

ophthalmology

SUMMARISED BY

AI pipeline

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100% · A

ORIGINAL PAPER

PMC10373963

MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway

Aging (Albany NY)

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ABSTRACT

Cataracts are the leading cause of blindness in the world. Age is a major risk factor for cataracts, and with increasing aging, the burden of cataracts will grow, but the exact details of cataractogenesis remain unclear. A recent study showed that microRNA-34a (MIR34A) is involved in the development of cataracts, but the underlying pathogenesis remains obscure. Here, our results of microRNA target prediction showed that hexokinase 1 (HK1) is one of the genes targeted by MIR34A. Based on this finding, we focused on the function of MIR34A and HK1 in the progress of cataracts, whereby the human lens epithelial cell line SRA01/04 and mouse lens were treated with MIR34A mimics and HK1 siRNA. We found that HK1 mRNA is a direct target of MIR34A, whereby the high expression of MIR34A in the cataract lens suppresses the expression of HK1. In vitro , the upregulation of MIR34A together with the downregulation of HK1 inhibits the proliferation, induces the apoptosis of SRA01/04 cells, and accelerates the opacification of mouse lenses via the HK1/caspase 3 signaling pathway. In summary, our study demonstrates that MIR34A modulates lens epithelial cell (LEC) apoptosis and cataract development through the HK1/caspase 3 signaling pathway.

CITATION EXPORT

Lujia Feng, Yantao Wei, Yimeng Sun et al.. MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway. Aging (Albany NY). (2023). https://doi.org/10.18632/aging.204854

AUTHORS

7 · 2 CENTRES

Lujia Feng, Yantao Wei, Yimeng Sun, Linbin Zhou, Shaowei Bi, Weirong Chen, Wu Xiang

1State Key Laboratory of Ophthalmology; Zhongshan Ophthalmic Center, Sun Yat-sen University; Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science; Guangdong Provincial Clinical Research Center for Ocular Diseases, Guangzhou 510060, China
2Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, Shenzhen 518040, China