Modulation of blood redox status by the progression of induced apical periodontitis in rats

Frontiers in Physiology · 10 authors, 3 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

The central finding was that the progression of the periapical lesion over 14 and 28 days was associated with changes in blood redox status, including a decrease in reduced glutathione and an increase in lipid peroxidation.

Full summary

619 CHARS

Systemic oxidative stress was assessed in blood at 14 and 28 days post-induction. Key results showed that at 14 days, there was a decrease in reduced glutathione and an increase in lipid peroxidation, alongside an established inflammatory lesion. By 28 days, these redox imbalances were more pronounced, correlating with significantly increased periapical bone resorption and inflammatory infiltrate. The findings suggest that systemic blood oxidative biochemistry is modulated by the duration and severity of the local periapical injury. The study implies a systemic biochemical response to localized dental infection.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10508187

Modulation of blood redox status by the progression of induced apical periodontitis in rats

Frontiers in Physiology

READ THE FULL PAPER

ABSTRACT

Abstract This study aimed to investigate if apical periodontitis in different periods changes systemic levels of the antioxidant and pro-oxidant parameters in Wistar rats. Twenty-four rats were randomly allocated into healthy animals, apical periodontitis at 14 days (AP14) and apical periodontitis at 28 days (AP28). The first mandibular molars were accessed in the AP groups, and the pulp chamber was exposed to the oral environment, inducing the apical lesion. After 14 and 28 days, the animals were anesthetized, euthanized, and hemimandibles were collected for micro-computed tomography (micro-CT) analysis to measure lesion volume, bone volume (BV), percent of bone to total tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular space (Tb.Sp). A histological examination of the remaining bone was also performed. Finally, blood samples were collected for oxidative biochemistry analysis, investigating glutathione (GSH), Trolox equivalent antioxidant capacity (TEAC), and lipid peroxidation (TBARS). The lesion volume was greater at 28 than at 14 days, as shown by micro-CT. AP14 and AP28 had decreased BV and Tb.Th, but only AP28 showed a reduction in BV/TV. Tb.N and Tb. Sp were increased in apical periodontitis at 28 days. In the histopathological analysis, AP14 had focal regions of moderate mononuclear inflammatory infiltrate, and AP28 had an intense inflammatory infiltrate with bacterial colonies. In the biochemical evaluation, GSH, TEAC, and TBARS were increased after 14 days. However, GSH returned to control levels, TEAC was similar to AP14, and TBARS increased significantly after 28 days. Therefore, the oxidative biochemistry response was modulated according to the progression of periapical damage. After 14 days, the organism could still react to the injury. However, at 28 days, the antioxidant response decreased, associated with an increase in TBARS. Keywords: periapical periodontitis, oxidative stress, wistar rats, microCT, inflammation

CITATION EXPORT

Deborah Ribeiro Frazão, Paulo Fernando Santos Mendes, Daiane Claydes Baia-da-Silva et al.. Modulation of blood redox status by the progression of induced apical periodontitis in rats. Frontiers in Physiology. (2023). https://doi.org/10.3389/fphys.2023.1214990

AUTHORS

10 · 3 CENTRES

Deborah Ribeiro Frazão, Paulo Fernando Santos Mendes, Daiane Claydes Baia-da-Silva, João Daniel Mendonça de Moura, Vinicius Ruan Neves dos Santos, José Mario Matos-Sousa, Gabriela de Souza Balbinot, Douglas Magno Guimarães, Fabrício Mezzomo Collares, Rafael Rodrigues Lima

1 Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Brazil
2 Dental Material Laboratory, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil
3 Centro Universitário do Para (CESUPA), Belém, Pará, Brazil