Moringa oleifera hydorethanolic leaf extract induced acute and sub-acute hepato-nephrotoxicity in female ICR-mice

Science Progress · 8 authors, 5 centres

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TL;DR

This preclinical study evaluated the acute and sub-acute oral toxicity of Moringa oleifera hydroethanolic leaf extract (MOHE) in female ICR mice.

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This preclinical study evaluated the acute and sub-acute oral toxicity of Moringa oleifera hydroethanolic leaf extract (MOHE) in female ICR mice. For the acute study, 10 mice received a single dose of 2000 mg/kg MOHE. MOHE contained glucomoringin, niaziminine, quercetin, and kaempferol. The single high dose caused mild anemia, stress leukogram, elevated AST and CK, and histological evidence of hepatic degeneration and renal tubular necrosis. Limitations include the small sample size and lack of power analysis. The study concludes MOHE's LD50 is greater than 2000 mg/kg, but it causes hepato-nephrotoxicity at these dose levels in mice.

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other

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preclinical research

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ORIGINAL PAPER

PMC10450773

Moringa oleifera hydorethanolic leaf extract induced acute and sub-acute hepato-nephrotoxicity in female ICR-mice

Science Progress

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ABSTRACT

Moringa oleifera (M. oleifera) Lam belongs to the family Moringaceae. It is an important multipurpose tree that is largely distributed globally and has been used almost in every aspect of traditional medicine for the treatment of various illnesses including cancers, diabetes mellitus, asthma, arthritis, etc. This study investigated the effects of oral acute and sub-acute administration of M. oleifera hydroethanolic leaf extract (MOHE) in ICR-mice. Its major phenolic compounds were also determined. Ten (10) female, 8-week old mice were grouped into control and treatment groups for acute toxicity study. A dose of 2000 mg/kg MOHE was given once to the treatment group via oral gavage. However, for the sub-acute toxicity study, 25 mice were grouped into groups A (control), B (125 mg/kg), C (250 mg/kg), D (500 mg/kg) and E (1000 mg/kg). MOHE was given via oral gavage to groups B, C, D and E daily for 28 days. Group A received only distilled water. The mice were sacrificed at the end of the experiments and samples were collected for evaluation. The results of the chemical profiling of MOHE revealed the presence of glucomoringin, niaziminine, quercetin and kaempferol as the major compounds. The treated mice in the acute toxicity study were slightly anaemic and showed evidence of stress leukogram. Moreover, a slight increase in creatinine, significant increases in AST and CK, hepatic degeneration and necrosis, none-obstructive sinusoidal dilatation, renal tubular necrosis, interstitial nephritis and renal interstitial oedema were observed. It is concluded that the LD 50 of MOHE is higher than 2000 mg/kg. However, oral administration of MOHE causes acute mild anaemia and moderate hepato-nephrotoxicity in ICR-mice. Its major phenolic compounds are glucomoringin, niaziminine, quercetin and kaempferol.

CITATION EXPORT

Abdullahi Aliyu, Mohd Rosly Shaari, Nurul Syahirah Ahmad Sayuti et al.. Moringa oleifera hydorethanolic leaf extract induced acute and sub-acute hepato-nephrotoxicity in female ICR-mice. Science Progress. (2021). https://doi.org/10.1177/00368504211004272

AUTHORS

8 · 5 CENTRES

Abdullahi Aliyu, Mohd Rosly Shaari, Nurul Syahirah Ahmad Sayuti, Farhan Hanif Reduan, Shanmugavelu Sithambaram, Noordin Mohamed Mustapha, Khozirah Shaari, Hazilawati Binti Hamzah

1Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
2Department of Veterinary Pathology, Usmanu Danfodiyo University, Sokoto, Nigeria
3Animal Science Research Centre, Malaysian Agricultural Research and Development Institute Headquarter, Serdang, Selangor, Malaysia
4Department of Chemistry, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
5Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia