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cohort·endocrinology, pulmonology, internal medicine, cardiology, preclinical research·PMC10048979

Muscle Lipid Oxidation Is Not Affected by Obstructive Sleep Apnea in Diabetes and Healthy Subjects

International Journal of Molecular Sciences · 7 authors, 3 centres

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TL;DR

It found that severe OSA did not affect muscle lipid oxidation or the expression of key fatty acid transport/metabolism proteins in either group.

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Using muscle biopsies and an ex vivo palmitate oxidation assay, along with measurements of key fatty acid transport/metabolism proteins (FATP4, CD36, CPT1), the study found no significant difference in lipid oxidation capacity or the expression of these proteins between patients with and without severe OSA. This held true for both the diabetic and healthy groups. The authors conclude that skeletal muscle lipid oxidation is not a mechanism through which OSA impairs whole-body glucose metabolism. Limitations include the ex vivo nature of the assay, measurements taken after awakening rather than during sleep, and a limited sample size.

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cohort

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endocrinology

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ORIGINAL PAPER

PMC10048979

Muscle Lipid Oxidation Is Not Affected by Obstructive Sleep Apnea in Diabetes and Healthy Subjects

International Journal of Molecular Sciences

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ABSTRACT

Abstract The molecular mechanisms linking obstructive sleep apnea (OSA) with type 2 diabetes mellitus (T2DM) remain unclear. This study investigated the effect of OSA on skeletal muscle lipid oxidation in nondiabetic controls and in type 2 diabetes (T2DM) patients. Forty-four participants matched for age and adiposity were enrolled: nondiabetic controls (control, n = 14), nondiabetic patients with severe OSA (OSA, n = 9), T2DM patients with no OSA (T2DM, n = 10), and T2DM patients with severe OSA (T2DM + OSA, n = 11). A skeletal muscle biopsy was performed; gene and protein expressions were determined and lipid oxidation was analyzed. An intravenous glucose tolerance test was performed to investigate glucose homeostasis. No differences in lipid oxidation (178.2 ± 57.1, 161.7 ± 22.4, 169.3 ± 50.9, and 140.0 ± 24.1 pmol/min/mg for control, OSA, T2DM, and T2DM+OSA, respectively; p > 0.05) or gene and protein expressions were observed between the groups. The disposition index, acute insulin response to glucose, insulin resistance, plasma insulin, glucose, and HBA 1 C progressively worsened in the following order: control, OSA, T2DM, and T2DM + OSA ( p for trend <0.05). No association was observed between the muscle lipid oxidation and the glucose metabolism variables. We conclude that severe OSA is not associated with reduced muscle lipid oxidation and that metabolic derangements in OSA are not mediated through impaired muscle lipid oxidation. Keywords: obstructive sleep apnea, type 2 diabetes mellitus, free fatty acids, lipid utilization, IVGTT, glucose intolerance, muscle metabolism, hypoxia

CITATION EXPORT

Zuzana Lattova, Lucie Slovakova, Andrea Plihalova et al.. Muscle Lipid Oxidation Is Not Affected by Obstructive Sleep Apnea in Diabetes and Healthy Subjects. International Journal of Molecular Sciences. (2023). https://doi.org/10.3390/ijms24065308

AUTHORS

7 · 3 CENTRES

Zuzana Lattova, Lucie Slovakova, Andrea Plihalova, Jan Gojda, Moustafa Elkalaf, Katerina Westlake, Jan Polak

1Department of Pathophysiology, Third Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic; [email protected] (Z.L.); [email protected] (L.S.); [email protected] (M.E.); [email protected] (K.W.)
2Department of Internal Medicine, University Hospital Kralovske Vinohrady, 100 34 Prague, Czech Republic; [email protected] (A.P.); [email protected] (J.G.)
3Centre for Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic